Department of Endodontics, Shiraz University of Medical Sciences, Shiraz, Iran.
Dent Traumatol. 2012 Apr;28(2):132-5. doi: 10.1111/j.1600-9657.2011.01054.x. Epub 2011 Sep 5.
The purpose of this study was to evaluate the ability of soymilk, powdered milk, and Hank's balanced salt solution (HBSS) to maintain human periodontal ligament (PDL) cell viability in vitro. PDL cells were obtained from extracted healthy third molars and cultured in Dulbecco's modified Eagles medium (DMEM). The cultures were exposed for 1, 2, 4, and 8 h to experimental solutions (tap water served as negative control and DMEM as positive control) at 37°C. The viable cells were then counted using the trypan blue exclusion technique. Data were analyzed by using one-way anova, post hoc Scheffe and two-way anova test. Statistical analysis showed that HBSS, powdered baby formula, and soymilk maintain cell viability equally well in different periods of times. Tap water cannot keep cells viable as well as other solutions. Soymilk and powdered baby formula can be recommended as suitable storage media for avulsed teeth for up to 8 h.
本研究旨在评估豆浆、配方奶粉和 Hank 平衡盐溶液（HBSS）在体外维持人牙周韧带（PDL）细胞活力的能力。PDL 细胞取自拔除的健康第三磨牙，并在 Dulbecco 改良 Eagle 培养基（DMEM）中培养。将培养物在 37°C 下分别暴露于实验溶液（自来水作为阴性对照，DMEM 作为阳性对照）1、2、4 和 8 小时。然后使用台盼蓝排除法计数存活细胞。使用单因素方差分析、事后 Scheffe 和双向方差分析对数据进行分析。统计分析表明，HBSS、婴儿配方奶粉和豆浆在不同时间段均能同样良好地维持细胞活力。自来水不如其他溶液能保持细胞活力。豆浆和配方奶粉可被推荐为 8 小时内保存离体牙的合适储存介质。
