Huang Cui-Hong, Chen Nan, Huang Chun-Xiao, Zhang Bao, Wu Meng, He Lei, Liu Hong, Tang Rong, Wang Wei-Min, Wang Huan-Ling
Key Lab of Freshwater Animal Breeding, Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Fishery, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China.
Freshwater Aquaculture Collaborative Innovation Center of Hubei Province, 430070, Wuhan, People's Republic of China.
Fish Physiol Biochem. 2017 Jun;43(3):863-873. doi: 10.1007/s10695-017-0341-8. Epub 2017 Mar 9.
MicroRNAs (miRNAs) are non-coding small RNAs showing both evolutionarily conserved and unique features and are involved in nearly all biological processes. In the present study, the role played by miR-462/731 cluster miRNAs in hypoxia response in Megalobrama amblycephala, an important freshwater fish, was investigated. The M. amblycephala miR-462/731 cluster locus and their 5' flanking sequences were sequenced and analyzed. In M. amblycephala and other teleost fish species, the mature sequences of miR-462 and miR-731 were identical and hypoxia-responsive elements (HREs) were identified upstream of the miR-462/731 loci. The two miRNAs were significantly induced in the liver, spleen, gill, muscle, and brain after hypoxia treatment. The expression of both miRNAs was also upregulated in cells that received treatment which mimicked hypoxia. Furthermore, reporter assay revealed that M. amblycephala HREs can be activated by hypoxia. Taken together, the 462/731 cluster may play a role in the regulation of the hypoxia response in M. amblycephala.
微小RNA（miRNA）是一类非编码小RNA，具有进化保守性和独特特征，几乎参与所有生物学过程。在本研究中，我们探究了重要淡水鱼鳙鱼中miR - 462/731簇miRNA在缺氧反应中的作用。对鳙鱼miR - 462/731簇基因座及其5'侧翼序列进行了测序和分析。在鳙鱼和其他硬骨鱼类中，miR - 462和miR - 731的成熟序列相同，且在miR - 462/731基因座上游鉴定到缺氧反应元件（HRE）。缺氧处理后，这两种miRNA在肝脏、脾脏、鳃、肌肉和脑中均显著上调。在模拟缺氧处理的细胞中，这两种miRNA的表达也上调。此外报告基因检测显示，鳙鱼HRE可被缺氧激活。综上所述，462/731簇可能在鳙鱼缺氧反应调控中发挥作用。
