Edwards S W, Lloyd D
Department of Biochemistry, University of Liverpool, England.
FEBS Lett. 1988 Jan 18;227(1):39-42. doi: 10.1016/0014-5793(88)81409-1.
The O2 affinity for O2.- generation (i.e. O2 tension at which 50% of the O2-saturated activity was observed) by rat neutrophils activated by the chemotactic peptide (N-formyl-methionyl-leucyl-phenylalanine) plus cytochalasin B was measured as 5.4 microM (+/- 1.4, n = 5). Similarly, the O2 tension at which cytochrome b was oxidized to 50% of its aerobic steady-state level in activated cells was 4.7 microM (+/- 1.0, n = 3): in non-activated cells the corresponding value for cytochrome b oxidation was 11.4 microM (+/- 2.8, n = 3). It is proposed that O2 depletion at inflammatory sites may limit oxidant generation by activated neutrophils and thus reduce oxidant damage to surrounding tissues.
通过趋化肽（N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸）加细胞松弛素B激活的大鼠中性粒细胞产生超氧阴离子（即观察到50%的氧饱和活性时的氧张力）的氧亲和力经测定为5.4微摩尔（±1.4，n = 5）。同样，在激活的细胞中细胞色素b被氧化至其需氧稳态水平的50%时的氧张力为4.7微摩尔（±1.0，n = 3）：在未激活的细胞中，细胞色素b氧化的相应值为11.4微摩尔（±2.8，n = 3）。有人提出，炎症部位的氧消耗可能会限制激活的中性粒细胞产生氧化剂，从而减少对周围组织的氧化损伤。
