反射干涉光谱法

Reflectometric Interference Spectroscopy.

作者信息

Proll Guenther, Markovic Goran, Fechner Peter, Proell Florian, Gauglitz Guenter

机构信息

Institute of Physical and Theoretical Chemistry, University of Tuebingen, Auf der Morgenstelle 18, 72076, Tuebingen, Germany.

Biametrics GmbH, Waldhaeuser Str. 64, 72076, Tuebingen, Germany.

出版信息

Methods Mol Biol. 2017;1571:207-220. doi: 10.1007/978-1-4939-6848-0_13.

DOI:10.1007/978-1-4939-6848-0_13

PMID:28281258

Abstract

Reflectometry is classified in comparison to the commercialized refractometric surface plasmon resonance. The advantages of direct optical detection depend on a sophisticated surface chemistry resulting negligible nonspecific binding and high loading with recognition sites at the biopolymer sensitive layer of the transducer. Elaborate details on instrumental realization and surface chemistry are discussed for optimum application of reflectometric interference spectroscopy (RIfS). A standard protocol for a binding inhibition assay is given. It overcomes principal problems of any direct optical detection technique.

摘要

与商业化的折射表面等离子体共振相比，反射测量法得到了分类。直接光学检测的优点取决于复杂的表面化学，其产生的非特异性结合可忽略不计，并且在换能器的生物聚合物敏感层上具有高识别位点负载。为了反射干涉光谱（RIfS）的最佳应用，讨论了仪器实现和表面化学的详细细节。给出了结合抑制测定的标准方案。它克服了任何直接光学检测技术的主要问题。