Gabriel Ellen Flávia Moreira, Garcia Paulo T, Evans Elizabeth, Cardoso Thiago M G, Garcia Carlos D, Coltro Wendell K T
Instituto de Química, Universidade Federal de Goiás, Campus Samambaia, 74690-900, Goiânia, GO, Brazil.
Department of Chemistry, Clemson University, Clemson, SC 29634, TX, 78249, USA.
Methods Mol Biol. 2017;1571:327-341. doi: 10.1007/978-1-4939-6848-0_20.
This chapter describes two different methodologies used to improve the analytical performance of colorimetric paper-based biosensors. Microfluidic paper-based analytical devices (μPADs) have been produced by a stamping process and CO laser ablation and modified, respectively, through an oxidation step and incorporation of silica nanoparticles on the paper structure. Both methods are employed in order to overcome the largest problem associated with colorimetric detection, the heterogeneity of the color distribution in the detection zones. The modification steps are necessary to improve the interaction between the paper surface and the selected enzymes. The enhanced performance has ensured reliability for quantitative analysis of clinically relevant compounds.
本章介绍了两种用于提高比色纸基生物传感器分析性能的不同方法。基于微流控的纸基分析装置（μPADs）分别通过冲压工艺和CO激光烧蚀制备，并分别通过氧化步骤和在纸结构中掺入二氧化硅纳米颗粒进行改性。采用这两种方法都是为了克服与比色检测相关的最大问题，即检测区域颜色分布的不均匀性。改性步骤对于改善纸表面与所选酶之间的相互作用是必要的。性能的提高确保了对临床相关化合物进行定量分析的可靠性。
