Wang Ling, Musile Giacomo, McCord Bruce R
Department of Chemistry and Biochemistry, Florida International University, Miami, FL, USA.
Department of Diagnostics and Public Health, Unit of Forensic Medicine, University of Verona, Verona, Italy.
Electrophoresis. 2018 Feb;39(3):470-475. doi: 10.1002/elps.201700254. Epub 2017 Sep 25.
A method utilizing paper microfluidics coupled with gold nanoparticles and two anticocaine aptamers has been developed to detect seized cocaine samples. The ready-to-use format involves the use of a paper strip that produces a color change resulting from the salt-induced aggregation of gold nanoparticles producing a visible color change indicating the presence of the drug. This format is specific for the detection of cocaine. The visual LOD for the method was 2.5 μg and the camera based LOD was 2.36 μg. The operation of the device is easy and rapid, and does not require extensive training or instrumentation. All of the materials utilized in the device are safe and environmental friendly. This device should prove a useful tool for the screening of forensic samples.
一种利用纸基微流体结合金纳米颗粒和两种抗可卡因适体来检测查获的可卡因样品的方法已被开发出来。这种即用型形式涉及使用纸条，盐诱导金纳米颗粒聚集会导致颜色变化，产生可见的颜色变化表明药物的存在。这种形式对可卡因的检测具有特异性。该方法的视觉检测限为2.5μg，基于相机的检测限为2.36μg。该装置操作简便、快速，不需要大量培训或仪器设备。该装置中使用的所有材料都是安全且环保的。该装置应被证明是一种用于法医样本筛查的有用工具。
