Wang Yuan-Kai, Yan Ya-Xian, Sun Jian-He
Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Methods Mol Biol. 2017;1571:435-447. doi: 10.1007/978-1-4939-6848-0_26.
Lateral flow immunoassays have been widely used in recent years for detection of toxins, heavy metals, and biomarkers. To improve the efficiency of individual lateral flow immunoassays, multiplex analytical strips play an important role in the detection of several important analytes. In this chapter, development of a dual lateral flow immunoassay is presented for detection of a variety of low molecular weight molecules. Various buffers, additives, and materials are introduced and evaluated. Depending on the analyte to be tested, the technique allows for selection of optimum buffers, additives, and other materials.
近年来，侧向流动免疫分析已广泛用于毒素、重金属和生物标志物的检测。为提高单个侧向流动免疫分析的效率，多重分析试纸条在几种重要分析物的检测中发挥着重要作用。在本章中，介绍了一种用于检测多种低分子量分子的双侧向流动免疫分析方法。引入并评估了各种缓冲液、添加剂和材料。根据待检测的分析物，该技术允许选择最佳的缓冲液、添加剂和其他材料。
