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利用暗场成像检测 DNA 诱导的金纳米颗粒聚集。

Detection of DNA induced gold nanoparticle aggregation with dark field imaging.

机构信息

Department of Advanced Materials Science, School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 227-8561, Japan.

出版信息

Chem Commun (Camb). 2013 Sep 4;49(68):7531-3. doi: 10.1039/c3cc44182b.

DOI:10.1039/c3cc44182b
PMID:23863889
Abstract

Dark field imaging was employed to visualize and quantify complementary DNA-induced gold nanoparticle aggregation at the single-particle level, with a detection limit of 100 fM DNA, which allows the highly sensitive detection of single nucleotide polymorphisms (SNPs). Results suggested that the aggregation process is consistent with the cluster-cluster model.

摘要

暗场成像被用于可视化和量化互补 DNA 诱导的金纳米颗粒在单粒子水平上的聚集，检测限为 100 fM DNA，这使得单核苷酸多态性 (SNP) 的高灵敏度检测成为可能。结果表明，聚集过程符合簇-簇模型。

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