Hashemzaei Mahmoud, Abdollahzadeh Mina, Iranshahi Mehrdad, Golmakani Ebrahim, Rezaee Ramin, Tabrizian Kaveh
J Complement Integr Med. 2017 Mar 1;14(1). doi: 10.1515/jcim-2016-0066.
Background Neuropathic pain (NP) is a common condition accompanied by nerve injury. To date, there is no definite treatment approved for this disorder. In addition, many drugs that are used for NP cause adverse reactions. Luteolin is a naturally occurring flavonoid with diverse pharmacological properties such as anti-inflammatory, antioxidant and anticancer. We sought to investigate luteolin effects on chronic, acute and neuropathic pain as well as its potential to increase morphine anti-nociceptive effects in mice. Methods Albino mice (20-25 g) were randomly divided into 14 groups (n=7) including morphine 1 mg/kg body weight +luteolin (5 mg/kg body weight), morphine (9 mg/kg body weight, i.p.), luteolin (2.5, 5 and 10 mg/kg body weight), imipramine 40 mg/kg body weight and normal saline (NS) (0.9 %) as vehicle and subjected to hot plate test. Formalin test was done in the following groups: NS, diclofenac sodium (10 mg/kg body weight, i.p.), morphine (9 mg/kg body weight, i.p.) and luteolin (2.5, 5 and 10 mg/kg body weight). Results Administration of luteolin single dose (5 and 10 mg/kg body weight) significantly reduced neuropathic pain ( p<0.05$\rm{p}<0.05$) in comparison to negative control. Anti-nociceptive effects of luteolin were comparable to imipramine as the standard positive control ( p<0.001$\rm{p}<0.001$). Co-administration of luteolin and morphine potentiated morphine 1 mg/kg body weight painkilling effects ( p<0.001$\rm{p}<0.001$). Conclusions Our results showed that luteolin alone reduces neuropathic pain. Furthermore, when co-administered with morphine 1 mg/kg body weight, luteolin potentiates morphine effects. Therefore, luteolin-morphine co-administration might be a valuable alternative for the conventional treatment.
神经性疼痛（NP）是一种伴随神经损伤的常见病症。迄今为止，尚无明确获批用于该疾病的治疗方法。此外，许多用于治疗NP的药物会引起不良反应。木犀草素是一种天然存在的黄酮类化合物，具有多种药理特性，如抗炎、抗氧化和抗癌作用。我们试图研究木犀草素对慢性、急性和神经性疼痛的影响，以及其增强小鼠吗啡镇痛作用的潜力。方法：将白化小鼠（20 - 25克）随机分为14组（每组n = 7），包括吗啡1毫克/千克体重 + 木犀草素（5毫克/千克体重）、吗啡（9毫克/千克体重，腹腔注射）、木犀草素（2.5、5和10毫克/千克体重）、丙咪嗪40毫克/千克体重以及作为溶媒的生理盐水（NS）（0.9%），并进行热板试验。在以下组中进行福尔马林试验：NS、双氯芬酸钠（10毫克/千克体重，腹腔注射）、吗啡（9毫克/千克体重，腹腔注射）和木犀草素（2.5、5和10毫克/千克体重）。结果：与阴性对照相比，单次给予木犀草素（5和10毫克/千克体重）可显著减轻神经性疼痛（p < 0.05）。木犀草素的镇痛作用与作为标准阳性对照的丙咪嗪相当（p < 0.001）。木犀草素与吗啡联合给药可增强吗啡1毫克/千克体重的止痛效果（p < 0.001）。结论：我们的结果表明，木犀草素单独使用可减轻神经性疼痛。此外，当与1毫克/千克体重的吗啡联合给药时，木犀草素可增强吗啡的作用。因此，木犀草素 - 吗啡联合给药可能是传统治疗的一种有价值的替代方法。
