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人抗菌肽SP-LL-37增强转基因水稻的抗病性。

SP-LL-37, human antimicrobial peptide, enhances disease resistance in transgenic rice.

作者信息

Lee In Hye, Jung Yu-Jin, Cho Yong Gu, Nou Ill Sup, Huq Md Amdadul, Nogoy Franz Marielle, Kang Kwon-Kyoo

机构信息

Department of Horticultural Life Science, Hankyong National University, Ansung, Korea.

Institute of Genetic Engineering, Hankyong National University, Ansung, Korea.

出版信息

PLoS One. 2017 Mar 10;12(3):e0172936. doi: 10.1371/journal.pone.0172936. eCollection 2017.

DOI:10.1371/journal.pone.0172936
PMID:28282452
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5345758/
Abstract

Human LL-37 is a multifunctional antimicrobial peptide of cathelicidin family. It has been shown in recent studies that it can serve as a host's defense against influenza A virus. We now demonstrate in this study how signal peptide LL-37 (SP-LL-37) can be used in rice resistance against bacterial leaf blight and blast. We synthesized LL-37 peptide and subcloned in a recombinant pPZP vector with pGD1 as promoter. SP-LL-37 was introduced into rice plants by Agrobacterium mediated transformation. Stable expression of SP-LL-37 in transgenic rice plants was confirmed by RT-PCR and ELISA analyses. Subcellular localization of SP-LL-37-GFP fusion protein showed evidently in intercellular space. Our data on testing for resistance to bacterial leaf blight and blast revealed that the transgenic lines are highly resistant compared to its wildtype. Our results suggest that LL-37 can be further explored to improve wide-spectrum resistance to biotic stress in rice.

摘要

人源LL-37是一种属于cathelicidin家族的多功能抗菌肽。近期研究表明，它可作为宿主抵御甲型流感病毒的防御物质。我们在本研究中展示了信号肽LL-37（SP-LL-37）如何用于水稻抗白叶枯病和稻瘟病。我们合成了LL-37肽，并将其亚克隆到以pGD1为启动子的重组pPZP载体中。通过农杆菌介导的转化将SP-LL-37导入水稻植株。通过RT-PCR和ELISA分析证实了SP-LL-37在转基因水稻植株中的稳定表达。SP-LL-37-GFP融合蛋白的亚细胞定位明显显示在细胞间隙。我们对白叶枯病和稻瘟病抗性测试的数据表明，与野生型相比，转基因株系具有高度抗性。我们的结果表明，LL-37可进一步探索用于提高水稻对生物胁迫的广谱抗性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fbb/5345758/bd45d28cb908/pone.0172936.g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fbb/5345758/bd45d28cb908/pone.0172936.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fbb/5345758/eaa6a1330a31/pone.0172936.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fbb/5345758/4f63f98215d3/pone.0172936.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fbb/5345758/70cb1c6c8eac/pone.0172936.g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fbb/5345758/37b5d77450e1/pone.0172936.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7fbb/5345758/bd45d28cb908/pone.0172936.g007.jpg

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