一种活体修饰生物蛋白 CP4-EPSPS 对致病真菌细胞的强效抗真菌功能。

Potent Antifungal Functions of a Living Modified Organism Protein, CP4-EPSPS, against Pathogenic Fungal Cells.

机构信息

Department of Chemical Engineering, Sunchon National University, Suncheon 38286, Republic of Korea.

LMO Team, National Institute of Ecology (NIE), Seocheon 33657, Republic of Korea.

出版信息

Molecules. 2023 May 24;28(11):4289. doi: 10.3390/molecules28114289.

DOI:10.3390/molecules28114289

PMID:37298765

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10254243/

Abstract

Various proteins introduced into living modified organism (LMO) crops function in plant defense mechanisms against target insect pests or herbicides. This study analyzed the antifungal effects of an introduced LMO protein, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) from sp. strain CP4 (CP4-EPSPS). Pure recombinant CP4-EPSPS protein, expressed in , inhibited the growth of human and plant fungal pathogens (, , , , , , and ), at minimum inhibitory concentrations (MICs) that ranged from 62.5 to 250 µg/mL. It inhibited fungal spore germination as well as cell proliferation on . Rhodamine-labeled CP4-EPSPS accumulated on the fungal cell wall and within intracellular cytosol. In addition, the protein induced uptake of SYTOX Green into cells, but not into intracellular mitochondrial reactive oxygen species (ROS), indicating that its antifungal action was due to inducing the permeability of the fungal cell wall. Its antifungal action showed cell surface damage, as observed from fungal cell morphology. This study provided information on the effects of the LMO protein, EPSPS, on fungal growth.

摘要

各种蛋白质被引入到活体修饰生物体（LMO）作物中，这些蛋白质在植物防御机制中起到了抵抗目标害虫或除草剂的作用。本研究分析了一种引入的 LMO 蛋白质的抗真菌效果，即来自 sp. 菌株 CP4（CP4-EPSPS）的 5-烯醇丙酮酰莽草酸-3-磷酸合酶（EPSPS）。在中表达的纯重组 CP4-EPSPS 蛋白，在最低抑菌浓度（MIC）为 62.5 至 250 µg/mL 的范围内，抑制了人类和植物真菌病原体（、、、、、、和）的生长。它抑制真菌孢子萌发以及在上的细胞增殖。罗丹明标记的 CP4-EPSPS 在真菌细胞壁和细胞内胞质溶胶中积累。此外，该蛋白诱导 SYTOX Green 进入细胞，但不进入细胞内线粒体活性氧（ROS），表明其抗真菌作用是由于诱导真菌细胞壁的通透性。其抗真菌作用显示出细胞表面损伤，如从真菌细胞形态观察到的。本研究提供了关于 LMO 蛋白质 EPSPS 对真菌生长影响的信息。