Danilov S M, Faerman A I, Martynov A V, Trakht I N
Institute of Experimental Cardiology, USSR Cardiology Research Center, Moscow.
Histochemistry. 1987;87(5):487-90. doi: 10.1007/BF00496822.
The localization of angiotensin-converting enzyme (ACE) in human tissues has been studied by the PAP-method with the use of monoclonal antibody 9 B9 against human lung ACE. The enzyme was detected on the surface of endothelial cells in lung, myocardium, liver, intestine and testis as well as in the epithelial cells of the kidney proximal tubules and intestine. The monoclonal antibody 9 B9 did not react with ACE in the epithelial cells of the testis seminiferous tubules. These data suggest that the antibody 9 B9 recognizes epitope which is shared by the ACE molecule of endothelial cells and renal and intestinal epithelial cells but is not present in testicular ACE, or is not accessible there to the antibody.
利用抗人肺血管紧张素转换酶（ACE）的单克隆抗体9 B9，通过过氧化物酶抗过氧化物酶法（PAP法）研究了ACE在人体组织中的定位。在肺、心肌、肝脏、肠道和睾丸的内皮细胞表面以及肾近端小管和肠道的上皮细胞中检测到了该酶。单克隆抗体9 B9与睾丸生精小管上皮细胞中的ACE不发生反应。这些数据表明，抗体9 B9识别的表位为内皮细胞、肾和肠上皮细胞的ACE分子所共有，但不存在于睾丸ACE中，或者在睾丸中该表位不能被抗体识别。
