Wang Jin, Yang Beibei, Zhong Jiatai, Yan Bo, Zhang Ke, Zhai Chunyang, Shiraishi Yukihide, Du Yukou, Yang Ping
College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, PR China.
School of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211, PR China.
J Colloid Interface Sci. 2017 Jul 1;497:172-180. doi: 10.1016/j.jcis.2017.03.011. Epub 2017 Mar 2.
A cubic Pd and reduced graphene oxide modified glassy carbon electrode (Pd/RGO/GCE) was fabricated to simultaneously detect dopamine (DA) and uric acid (UA) by cyclic voltammetry (CV) and different pulse voltammetry (DPV) methods. Compared with Pd/GCE and RGO/GCE, the Pd/RGO/GCE exhibited excellent electrochemical activity in electrocatalytic behaviors. Performing the Pd/RGO/GCE in CV measurement, the well-defined oxidation peak potentials separation between DA and UA reached to 145mV. By using the differential pulse voltammetry (DPV) technique, the calibration curves for DA and UA were found linear with the concentration range of 0.45-421μM and 6-469.5μM and the detection limit (S/N =3) were calculated to be 0.18μM and 1.6μM, respectively. Furthermore, the Pd/RGO/GCE displayed high selectivity when it was applied into the determination of DA and UA even though in presence of high concentration of interferents. Additionally, the prepared electrochemical sensor of Pd/RGO/GCE demonstrated a practical feasibility in rat urine and serum samples determination.
制备了一种立方钯和还原氧化石墨烯修饰的玻碳电极（Pd/RGO/GCE），通过循环伏安法（CV）和差分脉冲伏安法（DPV）同时检测多巴胺（DA）和尿酸（UA）。与Pd/GCE和RGO/GCE相比，Pd/RGO/GCE在电催化行为中表现出优异的电化学活性。在CV测量中使用Pd/RGO/GCE时，DA和UA之间明确的氧化峰电位分离达到145mV。通过差分脉冲伏安法（DPV）技术，发现DA和UA的校准曲线在0.45 - 421μM和6 - 469.5μM的浓度范围内呈线性，计算出的检测限（S/N = 3）分别为0.18μM和1.6μM。此外，即使在存在高浓度干扰物的情况下，将Pd/RGO/GCE应用于DA和UA的测定时，它也表现出高选择性。另外，所制备的Pd/RGO/GCE电化学传感器在大鼠尿液和血清样品测定中显示出实际可行性。
