Department of Biomedical Engineering Graduate Program, Izmir Katip Celebi University, Izmir, Turkey.
Department of Engineering Sciences, Izmir Katip Celebi University, Izmir, Turkey.
Anal Sci. 2024 Feb;40(2):301-308. doi: 10.1007/s44211-023-00459-6. Epub 2023 Nov 16.
Here, screen-printed carbon electrodes (SPCEs) were modified with ultrafine and mainly mono-disperse sea urchin-like tungsten oxide (SUWO) nanostructures synthesized by a simple one-pot hydrothermal method for non-enzymatic detection of dopamine (DA) and uric acid (UA) in synthetic urine. Sea urchin-like nanostructures were clearly observed in scanning electron microscope images and WO composition was confirmed with XRD, Raman, FTIR and UV-Vis spectrophotometer. Modification of SPCEs with SUWO nanostructures via the drop-casting method clearly reduced the R value of the electrodes, lowered the ∆Ep and enhanced the DA oxidation current due to high electrocatalytic activity. As a result, SUWO/SPCEs enabled highly sensitive non-enzymatic detection of DA (LOD: 51.4 nM and sensitivity: 127 µA mM cm) and UA (LOD: 253 nM and sensitivity: 55.9 µA mM cm) at low concentration. Lastly, SUWO/SPCEs were tested with synthetic urine, in which acceptable recoveries for both molecules (94.02-105.8%) were obtained. Given the high selectivity, the sensor has the potential to be used for highly sensitive simultaneous detection of DA and UA in real biological samples.
在此，通过简单的一锅水热法合成超细微且主要单分散的海胆状氧化钨（SUWO）纳米结构来修饰丝网印刷碳电极（SPCE），用于在合成尿液中对多巴胺（DA）和尿酸（UA）进行非酶检测。在扫描电子显微镜图像中可以清楚地观察到海胆状纳米结构，并用 XRD、拉曼、FTIR 和紫外可见分光光度计证实 WO 组成。通过滴涂法将 SUWO 纳米结构修饰到 SPCE 上，由于具有高电催化活性，明显降低了电极的 R 值，降低了 ∆Ep，并增强了 DA 氧化电流。结果，SUWO/SPCE 能够在低浓度下对 DA（LOD：51.4 nM 和灵敏度：127 µA mM cm）和 UA（LOD：253 nM 和灵敏度：55.9 µA mM cm）进行高灵敏度的非酶检测。最后，SUWO/SPCE 用合成尿液进行了测试，两种分子的回收率（94.02-105.8%）都在可接受范围内。鉴于其高选择性，该传感器有望用于在真实生物样品中对 DA 和 UA 进行高灵敏度的同时检测。
