用于检测牛1型疱疹病毒血清抗体的快速、灵敏、竞争性血清学酶联免疫吸附测定法。
Rapid, sensitive, competitive serologic enzyme-linked immunosorbent assay for detecting serum antibodies to bovine herpesvirus type 1.
作者信息
Riegel C A, Ayers V K, Collins J K
机构信息
Diagnostic Laboratory, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523.
出版信息
J Clin Microbiol. 1987 Dec;25(12):2418-21. doi: 10.1128/jcm.25.12.2418-2421.1987.
An enzyme-linked immunosorbent assay (ELISA) for the detection of cattle antibodies to bovine herpesvirus type 1 was developed on the basis of competition between serum antibody and a virus-neutralizing mouse monoclonal antibody. The assay showed improved sensitivity over the virus neutralization (VN) test and over an enhanced VN test in which incubation of antibody-virus mixtures was carried out for 24 h. With the ELISA, antibodies in sera from experimentally infected cattle were detected earlier after infection and showed more rapid increases in levels. A comparison of the ELISA with the VN tests by using a set of 85 field sera with low levels of antibodies demonstrated that the ELISA was the most sensitive test, detecting 10 positive serum samples that were negative by the VN tests. The ELISA was inexpensive, rapid, and highly reproducible and showed a significant improvement in sensitivity over VN tests.
基于血清抗体与病毒中和小鼠单克隆抗体之间的竞争关系，开发了一种用于检测牛抗1型牛疱疹病毒抗体的酶联免疫吸附测定（ELISA）法。该测定法比病毒中和（VN）试验以及抗体 - 病毒混合物孵育24小时的增强型VN试验具有更高的灵敏度。使用ELISA法，可在实验感染牛后更早地检测到血清中的抗体，且抗体水平升高更快。通过使用一组85份低水平抗体的现场血清将ELISA法与VN试验进行比较，结果表明ELISA法是最灵敏的检测方法，检测出了10份VN试验呈阴性的阳性血清样本。ELISA法成本低廉、速度快且具有高度可重复性，与VN试验相比，灵敏度有显著提高。
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