一种针对SV40大T抗原的单克隆抗体可标记JC病毒转化细胞以及感染了JC病毒的进行性多灶性白质脑病（PML）脑组织中的一种核抗原。

A monoclonal antibody to SV40 large T-antigen labels a nuclear antigen in JC virus-transformed cells and in progressive multifocal leukoencephalopathy (PML) brain infected with JC virus.

作者信息

Stoner G L, Ryschkewitsch C F, Walker D L, Soffer D, Webster H D

机构信息

Laboratory of Experimental Neuropathology, NINCDS, Bethesda, MD 20892.

出版信息

J Neuroimmunol. 1988 Mar;17(4):331-45. doi: 10.1016/0165-5728(88)90124-5.

DOI:10.1016/0165-5728(88)90124-5

PMID:2828425

Abstract

Thirty monoclonal antibodies to SV40 large T-antigen were tested for reactivity on the JC virus-transformed hamster glial cell line known as HJC-15. Two of them (PAb 416 and PAb 108) detected a nuclear antigen in both SV40-transformed CCL 75.1 cells and in HJC-15 cells, but not in control cells lacking T-antigen. These same antibodies also labeled a nuclear antigen in hamster tumor tissue derived from HJC-15 cells. In addition, the monoclonal antibody PAb 416 detected a nuclear antigen in progressive multifocal leukoencephalopathy (PML) tissue infected with JC virus, but not in normal brain tissue or tissue from other neurological diseases. Staining by PAb 416 was reduced by prior incubation with hamster anti-JCV tumor serum, suggesting that the polyclonal antiserum to JCV T-antigen may compete for an epitope at or near the PAb 416 binding site.

摘要

针对猿猴空泡病毒40（SV40）大T抗原的30种单克隆抗体，在名为HJC - 15的JC病毒转化的仓鼠神经胶质细胞系上进行了反应性测试。其中两种抗体（PAb 416和PAb 108）在SV40转化的CCL 75.1细胞和HJC - 15细胞中均检测到一种核抗原，但在缺乏T抗原的对照细胞中未检测到。同样这些抗体也标记了源自HJC - 15细胞的仓鼠肿瘤组织中的一种核抗原。此外，单克隆抗体PAb 416在感染JC病毒的进行性多灶性白质脑病（PML）组织中检测到一种核抗原，但在正常脑组织或其他神经疾病的组织中未检测到。用仓鼠抗JCV肿瘤血清预先孵育后，PAb 416的染色减弱，这表明针对JCV T抗原的多克隆抗血清可能在PAb 416结合位点或其附近竞争一个表位。