Department of Biochemical Engineering, College of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, Jiangsu, China.
Department of Biochemical Engineering, College of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, Jiangsu, China.
Bioresour Technol. 2017 Jun;233:284-290. doi: 10.1016/j.biortech.2017.02.106. Epub 2017 Feb 27.
An efficient strategy was developed in current work for biochemical conversion of carbohydrates of corn stover into monosaccharides. Corn stover was first presoaked in mild alkaline solution (1% NaS) under 40°C for 4h, after which about 35.3% of the lignin was successfully removed while the specific surface area was notably enlarged. Then the presoaked solids were subjected to organosolv pretreatment that employed 20% methanol with an addition of 0.2% HCl as catalyst at 160°C for 20min, and the maximum total sugar yield of the pretreated corn stover achieved was 98.6%. The intact structure of corn stover was disrupted by this two-step process, which resulted in a porous but crystalline structure of the regenerated solids that were mainly composed of cellulose. The enlarged specific surface area and increased accessibility made the regenerated solids highly digestible by a moderate enzyme loading.
在当前的工作中，开发了一种将玉米秸秆中的碳水化合物生化转化为单糖的有效策略。玉米秸秆首先在 40°C 下用温和的碱性溶液（1% NaS）预浸泡 4 小时，在此过程中约 35.3%的木质素被成功去除，同时比表面积显著增大。然后，将预浸泡的固体进行有机溶剂预处理，在 160°C 下用 20%甲醇和 0.2%盐酸作为催化剂处理 20 分钟，预处理后的玉米秸秆的最大总糖得率为 98.6%。两步法破坏了玉米秸秆的完整结构，导致再生固体呈现多孔但结晶的结构，主要由纤维素组成。较大的比表面积和增加的可及性使得再生固体在中等酶负荷下具有较高的消化率。
