Tan Bibo, Li Yong, Shi Xiaoming, Fan Liqiao, Zhao Qun, Liu Yanli, Tan Ming, Liu Qingwei, Jia Nan
Third Department of Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, 050011, China.
Third Department of Surgery, The Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang, 050011, China.
Pathol Res Pract. 2017 May;213(5):435-440. doi: 10.1016/j.prp.2017.01.028. Epub 2017 Feb 24.
Vav3 is associated with tumor growth, apoptosis, invasion, metastasis and angiogenesis. In this study, we detected the expression of Vav3 in gastric cancer tissues, and explored its role in invasion, metastasis and prognosis of gastric cancer. Vav3, MMP-2, MMP-9, TIMP-1 and TIMP-2 in primary lesion and pericarcinous tissues were tested with Immunohistochemistry and Western blot. Results showed a higher expression of Vav3 in primary lesion than in pericarcinous tissue, and the expression of Vav3 was significantly correlated with MMP-2, MMP-9 and TIMP-1 in gastric cancer tissues. Overexpression of Vav3 was associated with poorer differentiation, advanced clinical stage, more significant infiltration depth, lymphatic metastasis, and perineural invasion. Results of Kaplan-Meier verified that overexpression of Vav3 was related to poorer prognosis and shorter survival time. Moreover, Cox proportional hazard model revealed that overexpression of Vav3 was an independent risk factor of prognosis for patients with gastric cancer. In all, we conclude that overexpression of Vav3 is an independent risk factor for prognosis of gastric cancer, and can be used as a prognostic indicator. This may be because that Vav3 could regulate genes which associated with the invasion and metastasis.
Vav3与肿瘤生长、凋亡、侵袭、转移及血管生成相关。在本研究中，我们检测了Vav3在胃癌组织中的表达，并探讨其在胃癌侵袭、转移及预后中的作用。采用免疫组织化学和蛋白质印迹法检测原发性病灶及癌旁组织中Vav3、基质金属蛋白酶-2（MMP-2）、基质金属蛋白酶-9（MMP-9）、金属蛋白酶组织抑制因子-1（TIMP-1）和金属蛋白酶组织抑制因子-2（TIMP-2）。结果显示，原发性病灶中Vav3的表达高于癌旁组织，且Vav3的表达与胃癌组织中的MMP-2、MMP-9和TIMP-1显著相关。Vav3的过表达与较差的分化程度、临床分期进展、浸润深度更显著、淋巴转移及神经周围浸润相关。Kaplan-Meier结果证实，Vav3的过表达与较差的预后及较短的生存时间相关。此外，Cox比例风险模型显示，Vav3的过表达是胃癌患者预后的独立危险因素。总之，我们得出结论，Vav3的过表达是胃癌预后的独立危险因素，可作为预后指标。这可能是因为Vav3可调节与侵袭和转移相关的基因。
