Tan Bi-Bo, Li Yong, Fan Li-Qiao, Zhao Qun, Liu Qing-Wei, Liu Yü, Wang Dong, Jia Nan
Department of General Surgery, The Fourth Affiliated Hospital, Hebei Medical University, Shijiazhuang, China.
Tumour Biol. 2017 May;39(5):1010428317698392. doi: 10.1177/1010428317698392.
Several studies have proved that Vav2 gene is associated with the carcinogenesis of some tumors, but the relationship between Vav2 gene and gastric cancer remains unclear. Purpose of this study is to detect the expression of Vav2 protein in gastric cancer tissues and to evaluate the clinical value of Vav2. Furthermore, both effect of Vav2 gene on invasion and metastasis of gastric cancer cells and its mechanism are investigated in vitro. Results showed that positive rate of Vav2 protein was significantly higher in gastric cancer tissues than in adjacent tissues and notably higher in metastatic lymph nodes than in gastric cancer tissues. Results of western blot were consistent with immunohistochemistry. Expression of Vav2 protein in gastric cancer tissues was related to degree of tumor differentiation, lymph node metastasis, and clinical stages. Inhibition of endogenous Vav2 in BGC823 cells led to significantly decreased cell activity, migration, and invasion ability in vitro, and expression of Rac1, MMP-2, and MMP-9 decreased, whereas expression of TIMP-1 increased. We concluded that Vav2 might promote invasion and metastasis of gastric cancer by regulating some invasion and metastasis-related genes.
多项研究已证明Vav2基因与某些肿瘤的发生有关，但Vav2基因与胃癌之间的关系仍不清楚。本研究的目的是检测Vav2蛋白在胃癌组织中的表达，并评估Vav2的临床价值。此外，在体外研究了Vav2基因对胃癌细胞侵袭和转移的影响及其机制。结果显示，Vav2蛋白在胃癌组织中的阳性率显著高于癌旁组织，在转移淋巴结中的阳性率明显高于胃癌组织。蛋白质印迹结果与免疫组织化学结果一致。Vav2蛋白在胃癌组织中的表达与肿瘤分化程度、淋巴结转移及临床分期有关。抑制BGC823细胞内源性Vav2导致体外细胞活性、迁移和侵袭能力显著降低，Rac1、MMP-2和MMP-9的表达降低，而TIMP-1的表达增加。我们得出结论，Vav2可能通过调节一些侵袭和转移相关基因来促进胃癌的侵袭和转移。
