Burt H M, Jackson J K
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada.
J Rheumatol. 1987 Oct;14(5):968-73.
The effects of incubation time and buffers of differing phosphate concentration on the ability of well characterized, synthetic triclinic calcium pyrophosphate dihydrate (CPPD) crystals to cause hemolysis of erythrocytes were determined. Maximum hemolysis was attained between 8-11 h; the percent hemolysis was 79% at a high crystal concentration of 50 mg/ml. The percent hemolysis values decreased with increasing phosphate concentration, which may have been due to adsorbed phosphate ions partially masking or protecting the erythrocyte membrane from a binding interaction with CPPD crystals. CPPD crystals possessed a significant negative surface potential, and we postulate that the CPPD crystal-membrane interaction may be mediated via an electrostatic mechanism.
研究了孵育时间以及不同磷酸盐浓度的缓冲液对特性明确的合成三斜晶型焦磷酸钙二水合物（CPPD）晶体引起红细胞溶血能力的影响。在8 - 11小时之间达到最大溶血；在50 mg/ml的高晶体浓度下，溶血百分比为79%。溶血百分比值随着磷酸盐浓度的增加而降低，这可能是由于吸附的磷酸根离子部分掩盖或保护了红细胞膜，使其免受与CPPD晶体的结合相互作用。CPPD晶体具有显著的负表面电位，我们推测CPPD晶体与膜的相互作用可能是通过静电机制介导的。
