Burt H M, Jackson J K
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, Canada.
Ann Rheum Dis. 1993 Aug;52(8):599-607. doi: 10.1136/ard.52.8.599.
Little is known about the effect on crystal induced neutrophil responses of the opsonisation of calcium pyrophosphate dihydrate (CPPD) (triclinic) crystals with components of serum and plasma. The purpose of this study was to determine the effects of precoating CPPD crystals with plasma, serum, complement depleted serum, and IgG on a full range of crystal induced neutrophil responses (calcium mobilisation, chemiluminescence, superoxide anion production, non-cytolytic lysosomal enzyme release, and leukotriene synthesis).
Crystals were precoated with IgG, serum, plasma, or complement depleted serum (heated at 56 degrees C), incubated with neutrophils and the responses monitored with time. Measurement of the extent of neutrophil association with crystals was based on monitoring the decrease in fluorescence intensity of supernatants when crystals and diphenylhexatriene labelled neutrophils were allowed to settle under gravity.
Precoating CPPD crystals with IgG, plasma, and serum significantly enhanced chemiluminescence, superoxide anion generation, increases in cytosolic free calcium levels, and non-cytolytic lysosomal enzyme release by neutrophils compared with uncoated CPPD crystals. The enhancement of neutrophil responses by crystals coated with complement depleted serum was less pronounced. The increased neutrophil responses induced by CPPD crystals coated with IgG might have been due to the observed increase in the association of IgG coated crystals with neutrophils.
These data show that there is a marked potentiation of all neutrophil responses to IgG, plasma, and serum coated CPPD crystals. It is suggested that the adsorption of synovial fluid proteins, including IgG and C3b, to CPPD crystals in vivo, results in the opsonised crystals becoming a potent neutrophil stimulant and inflammatory agent.
关于血清和血浆成分对二水焦磷酸钙（CPPD）（三斜晶系）晶体调理作用后对晶体诱导的中性粒细胞反应的影响，目前了解甚少。本研究的目的是确定用血浆、血清、补体缺失血清和IgG预包被CPPD晶体对一系列晶体诱导的中性粒细胞反应（钙动员、化学发光、超氧阴离子产生、非溶细胞性溶酶体酶释放和白三烯合成）的影响。
晶体用IgG、血清、血浆或补体缺失血清（56℃加热）预包被，与中性粒细胞孵育，并随时间监测反应。中性粒细胞与晶体结合程度的测量基于监测当晶体和二苯基己三烯标记的中性粒细胞在重力作用下沉淀时上清液荧光强度的降低。
与未包被的CPPD晶体相比，用IgG、血浆和血清预包被CPPD晶体显著增强了中性粒细胞的化学发光、超氧阴离子生成、胞质游离钙水平升高和非溶细胞性溶酶体酶释放。补体缺失血清包被的晶体对中性粒细胞反应的增强作用不太明显。IgG包被的CPPD晶体诱导的中性粒细胞反应增加可能是由于观察到IgG包被的晶体与中性粒细胞的结合增加。
这些数据表明，所有中性粒细胞对IgG、血浆和血清包被的CPPD晶体的反应均有显著增强。提示体内滑膜液蛋白（包括IgG和C3b）吸附到CPPD晶体上，导致调理后的晶体成为有效的中性粒细胞刺激剂和炎症介质。
