Lao Lifeng, Shen Jia, Tian Haijun, Zhong Guibin, Murray Samuel S, Wang Jeffrey C
Department of Orthopaedic Surgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Department of Orthopaedic Surgery, University of California at Los Angeles (UCLA), Los Angeles, CA, USA.
Environ Toxicol Pharmacol. 2017 Apr;51:51-55. doi: 10.1016/j.etap.2017.03.001. Epub 2017 Mar 4.
Several studies have shown that secreted phosphoprotein 24kD (Spp24) inhibits tumor growth. However, the effects of spp24 on hepatocellular carcinoma are not quite clear. In this study, we observed the inhibitory effect of spp24 on hepatocellular carcinoma in vivo. A subcutaneous hepatocellular carcinoma mice model was established by using Hep G2 cells. After sacrifice at day 40, tumor growth was assessed and tumor cell apoptosis and tumor cells proliferation were assessed by TUNEL assay and immunochemical analysis, respectively. BMP2 slightly stimulated the subcutaneous tumor growth compared with the control. Spp24 significantly inhibited the tumor growth and also abolished the BMP2-induced tumor growth (p<0.05). TUNEL assay and immunochemical analysis further showed that spp24 could enhance tumor cell apoptosis and inhibit cell proliferation (p<0.01). Our data show that spp24 can inhibit the growth of hepatocellular carcinoma. Spp24 may have great potential for cancer treatment.
多项研究表明，分泌型磷蛋白24kD（Spp24）可抑制肿瘤生长。然而，Spp24对肝细胞癌的影响尚不完全清楚。在本研究中，我们观察了Spp24在体内对肝细胞癌的抑制作用。利用Hep G2细胞建立了皮下肝细胞癌小鼠模型。在第40天处死小鼠后，评估肿瘤生长情况，并分别通过TUNEL法和免疫化学分析评估肿瘤细胞凋亡和肿瘤细胞增殖情况。与对照组相比，BMP2轻微刺激了皮下肿瘤生长。Spp24显著抑制肿瘤生长，并且消除了BMP2诱导的肿瘤生长（p<0.05）。TUNEL法和免疫化学分析进一步表明，Spp24可增强肿瘤细胞凋亡并抑制细胞增殖（p<0.01）。我们的数据表明，Spp24可抑制肝细胞癌的生长。Spp24在癌症治疗方面可能具有巨大潜力。
