Ishikawa Shintaro, Ishikawa Takako, Tezuka Chiaki, Asano Kazuhito, Sunagawa Masataka, Hisamitsu Tadashi
Department of Physiology, School of Medicine, Showa University, Tokyo, Japan.
Evid Based Complement Alternat Med. 2017;2017:6054706. doi: 10.1155/2017/6054706. Epub 2017 Feb 12.
. Medical care for Japanese cancer patients includes Western and Kampo medicines, and treatments with juzentaihoto (JTT) reportedly prevent cancer metastasis and recurrence. In this study, we examined the effects of JTT on natural killer (NK) cell activity and metastasis in combined treatments with anti-PD-1 antibody in a mouse model of melanoma metastasis. . C57BL/6 male mice were intravenously injected with B16 melanoma cells (B16 cell) and were given chow containing 3% JTT. In subsequent in vivo experiments, we assessed serum cytokine levels and tumor colony formation in the lungs. Additionally, we assessed NK cell activity in ex vivo experiments. . JTT significantly suppressed B16 cell metastasis, whereas injection of anti-asialo-GM1 antibody into mice abrogated the inhibitory actions of JTT. JTT significantly increased interleukin- (IL-) 12 and interferon- (IFN-) levels in serum and induced NK cell activity. It increased the inhibitory actions of the anti-PD-1 antibody on B16 cell metastasis. . These data suggest that JTT inhibits B16 cell metastasis by inducing NK cell activity. Additionally, combination therapy with JTT and anti-PD-1 antibody increased treatment response rates for B16 melanoma.
日本癌症患者的医疗护理包括西药和汉方药物，据报道，十全大补汤（JTT）治疗可预防癌症转移和复发。在本研究中，我们在黑色素瘤转移小鼠模型中研究了JTT在与抗PD - 1抗体联合治疗时对自然杀伤（NK）细胞活性和转移的影响。
将C57BL / 6雄性小鼠静脉注射B16黑色素瘤细胞（B16细胞），并给予含3％JTT的食物。在随后的体内实验中，我们评估了血清细胞因子水平和肺部肿瘤集落形成。此外，我们在体外实验中评估了NK细胞活性。
JTT显著抑制B16细胞转移，而向小鼠注射抗去唾液酸GM1抗体则消除了JTT的抑制作用。JTT显著提高血清中白细胞介素 - （IL - ）12和干扰素 - （IFN - ）水平，并诱导NK细胞活性。它增强了抗PD - 1抗体对B16细胞转移的抑制作用。
这些数据表明，JTT通过诱导NK细胞活性来抑制B16细胞转移。此外，JTT与抗PD - 1抗体的联合治疗提高了B16黑色素瘤的治疗反应率。
