Department of Physiology, School of Medicine, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan.
Evid Based Complement Alternat Med. 2012;2012:945714. doi: 10.1155/2012/945714. Epub 2011 Oct 27.
Juzentaihoto (JTT) is well known to be one of Japanese herbal medicines, and used for the supplemental therapy of cancer patients with remarkable success. The present study, therefore, was undertaken to examine the possible therapeutic mechanisms of JTT on cancer using B16 melanoma cell (B16 cell)/experimental mouse system. JTT was well mixed with rodent chow at 3.0% concentrations, and was administered orally ad libitum. Administration of JTT was started one week before tumor cell injection and continued throughout the experiment. Administration of JTT into mice significantly inhibited tumor metastasis in lungs after intravenous injection of 2 × 10(5) B16 cells in a volume of 50 μL. JTT also significantly suppressed enlargement of tumor size in hind footpad after the subcutaneous injection of 2 × 10(5) (50 μL) B16 cells. In the second part of experiments, the chamber that containing B16 cells was buried in the murine back. In JTT administrated group, vascular endothelial growth factor (VEGF) of chamber internal fluid significantly decreased, and vascularization of chamber circumference was also inhibited. These results strongly suggest that oral administration of JTT caused decrease in the generation of VEGF, which is responsible for vascularization, and results in inhibition of B16 cell metastasis.
救心丹（JTT）是一种日本草药，常用于癌症患者的辅助治疗，并取得显著成效。因此，本研究采用 B16 黑色素瘤细胞（B16 细胞）/实验鼠系统，探讨 JTT 治疗癌症的可能机制。JTT 与鼠粮以 3.0%的浓度充分混合，自由口服给药。在肿瘤细胞注射前一周开始给药，并持续整个实验过程。JTT 给药显著抑制了静脉注射 2×10(5)个 B16 细胞（体积为 50μL）后肺部的肿瘤转移。JTT 还显著抑制了皮下注射 2×10(5)个（50μL）B16 细胞后后脚掌肿瘤大小的增大。在实验的第二部分，含有 B16 细胞的小室被埋在鼠背上。在 JTT 给药组中，小室内液的血管内皮生长因子（VEGF）显著减少，小室周围的血管化也受到抑制。这些结果强烈表明，JTT 的口服给药导致了负责血管生成的 VEGF 的产生减少，从而抑制了 B16 细胞的转移。
