Liu Yufang, Zhao Fengchun, Liu Jiye, Wang Huimin, Han Xiao, Zhang Yongxin, Yang Zhengyou
Key Laboratory for Agricultural Microbiology, Department of Microbiology College of Life Science, Shandong Agricultural University, Taian, 271000, China.
Curr Microbiol. 2017 May;74(5):623-631. doi: 10.1007/s00284-017-1230-1. Epub 2017 Mar 13.
High cholesterol level in serum is a major factor of influence for coronary heart disease. The cholesterol-lowering ability of lactic acid bacteria (LAB) without side effects makes them more and more attractive. Seventy-nine strains of LAB isolated from fermented food were screened in vitro for their ability to assimilate cholesterol. Then, ten strains which exhibited higher ability of cholesterol assimilation were investigated with the characteristics of acidic resistance, bile salt tolerance, and cell adhesion. According to the results, the best strain LP96 was picked out, and used to evaluate its effects on the high-cholesterol diet-fed rats. The results demonstrated that the levels of serum triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol, and liver TC and TG were reduced significantly in the groups that received the strain LP96 solution compared with the model group, and that the serum high-density lipoprotein cholesterol levels were increased without any significant difference. Furthermore, LP96 also showed good antioxidative activity and improvement of intestinal microbial balance in the rats. Thus, LP96 may be a promising probiotics with potential cholesterol-lowering ability.
血清中高胆固醇水平是影响冠心病的主要因素。乳酸菌（LAB）具有降低胆固醇的能力且无副作用，这使其越来越具有吸引力。从发酵食品中分离出79株乳酸菌，在体外筛选它们同化胆固醇的能力。然后，对10株表现出较高胆固醇同化能力的菌株进行耐酸性、耐胆盐性和细胞黏附特性研究。根据结果，选出最佳菌株LP96，并用于评估其对高胆固醇饮食喂养大鼠的影响。结果表明，与模型组相比，接受菌株LP96溶液的组中血清甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇以及肝脏TC和TG水平显著降低，血清高密度脂蛋白胆固醇水平升高但无显著差异。此外，LP96在大鼠中还表现出良好的抗氧化活性并改善了肠道微生物平衡。因此，LP96可能是一种有前景的具有潜在降胆固醇能力的益生菌。
