Faculty of Applied Sciences, Tunku Abdul Rahman University College, Kuala Lumpur, Malaysia.
Institute of Biosience, Universiti Putra Malaysia, Selangor, Malaysia.
J Appl Microbiol. 2020 Nov;129(5):1374-1388. doi: 10.1111/jam.14678. Epub 2020 Jun 10.
To determine the mechanism underlying the serum cholesterol reduction effect by probiotics isolated from local fermented tapioca (Tapai).
Lactic acid bacteria strains were isolated and examined for acid tolerance, bile salt resistance and hypocholesterolemic properties. Among the isolates, Lactobacillus plantarum TAR4 showed the highest cholesterol reduction ability (48·01%). The focus in the in vivo trial was to elucidate the cholesterol balance from findings pertaining to serum cholesterol reduction in rat model fed with high fat diet via oral administration. Rats fed with high-cholesterol diet supplemented with Lact. plantarum TAR4 showed significant reduction in serum total cholesterol (29·55%), serum triglyceride (45·31%) and liver triglyceride (23·44%) as compared to high-cholesterol diet (HCD) group. There was a significant increment in faecal triglyceride (45·83%) and faecal total bile acid (384·95%) as compared to HCD group.
The findings showed that probiotic Lact. plantarum TAR4 supplementation reduced the absorption of bile acids for enterohepatic recycling and increased the catabolism of cholesterol to bile acids and not by suppressing the rate of cholesterol synthesis.
Probiotic supplements could provide a new nonpharmacological alternative to reduce cardiovascular risk factors.
确定从当地发酵木薯（Tapioca）中分离出的益生菌降低血清胆固醇的作用机制。
分离并检测了乳酸菌的耐酸性、耐胆盐性和降胆固醇特性。在分离株中，植物乳杆菌 TAR4 显示出最高的胆固醇降低能力（48.01%）。在体内试验中，重点是通过口服高胆固醇饮食的大鼠模型来阐明胆固醇平衡，从血清胆固醇降低的发现中得出结论。与高胆固醇饮食（HCD）组相比，补充植物乳杆菌 TAR4 的大鼠血清总胆固醇（29.55%）、血清甘油三酯（45.31%）和肝甘油三酯（23.44%）显著降低。与 HCD 组相比，粪便甘油三酯（45.83%）和粪便总胆汁酸（384.95%）显著增加。
研究结果表明，益生菌植物乳杆菌 TAR4 的补充减少了胆汁酸的吸收，以进行肠肝循环，并增加了胆固醇向胆汁酸的代谢，而不是通过抑制胆固醇合成的速度。
益生菌补充剂可能为降低心血管危险因素提供一种新的非药物替代方法。
