Javed Saman, Latif Zakia, Javed Ghulam Ayesha, Munir Aneela, Mushtaq Maria, Hameed Mamoona, Arshad Muhammad, Arshad Najma
Institute of Zoology, University of the Punjab, Lahore, 54590, Pakistan.
Department of Biology, Lahore Garrison University, Lahore, Pakistan.
Curr Microbiol. 2025 Apr 1;82(5):222. doi: 10.1007/s00284-025-04173-6.
The current study explored cholesterol-lowering potential and the underlying mechanism (biliary cholesterol excretion) of a prebiotic (mannitol), probiotic (Weissella confusa MW051433), and their synbiotic (mannitol + W. confusa MW051433) in high fat diet induced hypercholesterolemic murine model. The experiment employed a randomized controlled design consisting of five groups: negative control, positive control and three treatment groups, with six rats in each group. Positive control and treatment groups were maintained on high fat (18.37%) diet throughout experimental period (60 days). The high-fat diet induced significant (p < 0.05) augmentation in total serum cholesterol, triglycerides, low-density lipoprotein and decrease in high-density lipoprotein within 15 days. By 60th day of experiment, it further led to elevation in liver cholesterol, and hepatic enzymes, and depletion in hemoglobin (35.85%), erythrocytes (34.78%), and hematocrit (27.31%) in PC group. ANOVA followed by Tukey's test revealed that treatment (day 15-60 of experiment) with W. confusa MW051433 and its synbiotic, significantly improved blood lipid profile, liver functions and led to remarkable increase in fecal cholesterol (70.48%, 107.85%) and cholic acid (123.21%, 100.91%) content, respectively. Histopathological findings, including hepatic lipid droplet count, adipocyte size, and cellular integrity, further indicated that the strain facilitated cholesterol reduction through biliary cholesterol excretion mechanism. While the synbiotic showed slight superiority in one parameter, the probiotic alone yielded comparable results. To our information, this is the first report on in vivo hypocholesterolemic efficacy of W. confusa MW051433. Further research exploring its role in cholesterol homeostasis through in silico modeling, and gene/protein expression, is recommended.
本研究探讨了益生元（甘露醇）、益生菌（困惑魏斯氏菌MW051433）及其合生元（甘露醇 + 困惑魏斯氏菌MW051433）在高脂饮食诱导的高胆固醇血症小鼠模型中的降胆固醇潜力及其潜在机制（胆汁胆固醇排泄）。实验采用随机对照设计，分为五组：阴性对照组、阳性对照组和三个治疗组，每组六只大鼠。阳性对照组和治疗组在整个实验期间（60天）维持高脂（18.37%）饮食。高脂饮食在15天内导致总血清胆固醇、甘油三酯、低密度脂蛋白显著升高（p < 0.05），高密度脂蛋白降低。到实验第60天时，阳性对照组进一步导致肝脏胆固醇、肝酶升高，血红蛋白（35.85%）、红细胞（34.78%）和血细胞比容（27.31%）减少。方差分析后进行Tukey检验显示，用困惑魏斯氏菌MW051433及其合生元治疗（实验第15 - 60天），显著改善了血脂谱、肝功能，并分别导致粪便胆固醇（70.48%，107.85%）和胆酸（123.21%，100.91%）含量显著增加。组织病理学结果，包括肝脂质滴计数、脂肪细胞大小和细胞完整性，进一步表明该菌株通过胆汁胆固醇排泄机制促进胆固醇降低。虽然合生元在一个参数上显示出轻微优势，但单独使用益生菌也产生了类似的结果。据我们所知，这是关于困惑魏斯氏菌MW051433体内降胆固醇功效的首次报道。建议通过计算机模拟以及基因/蛋白质表达进一步研究其在胆固醇稳态中的作用。
