Wang Yan, Kong Desheng, Xu Weisi, Li Fan, Liang Shu, Feng Yi, Zhang Fujie, Shao Yiming, Ma Liying
1 State Key Laboratory of Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention , Beijing, People's Republic of China .
2 Clinical and Research Center of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University , Beijing, People's Republic of China .
AIDS Res Hum Retroviruses. 2017 Jun;33(6):621-628. doi: 10.1089/aid.2017.0002. Epub 2017 Mar 13.
We report in this study a novel HIV-1 unique recombinant virus (XC2014EU01) isolated from an HIV-positive man who infected through heterosexual sex in Sichuan, China. The near full-length genome analyses showed that XC2014EU01 harbored one subtype B segment in pol region and two subtype C segments in gag-pol region in a CRF01_AE backbone. The unique mosaic structure was distinctly different from the other CRF01_AE/B'/C recombinant forms reported. Phylogenetic tree analyses revealed that the subtype B region originated from a Thailand subtype B' lineage, the subtype C regions were from an India C lineage, and the backbone was from CRF01_AE. XC2014EU01 was still identified as CCR5-tropic, and plasma of XC2014EU01 infected person had the media neutralizing activity. The emergence of XC2014EU01 may increase the complexity of the HIV-1 epidemic among high-risk populations and the difficulty of vaccine research and development.
我们在本研究中报告了一种新型的HIV-1独特重组病毒（XC2014EU01），它是从一名在中国四川通过异性性行为感染的HIV阳性男子体内分离出来的。近乎全长的基因组分析表明，XC2014EU01在CRF01_AE主干的pol区域含有一个B亚型片段，在gag-pol区域含有两个C亚型片段。这种独特的镶嵌结构与报道的其他CRF01_AE/B'/C重组形式明显不同。系统发育树分析显示，B亚型区域起源于泰国B'谱系，C亚型区域来自印度C谱系，主干来自CRF01_AE。XC2014EU0仍然被鉴定为CCR5嗜性，XC2014EU01感染者的血浆具有中等中和活性。XC2014EU01的出现可能会增加高危人群中HIV-1流行的复杂性以及疫苗研发的难度。
