Gui Tao, Zhao Jin, Sun Changrong, Chen Lin, Liu Yongjian, Zheng Chenli, Li Hanping, Li Tianyi, Bao Zuoyi, Wang Xiaolin, Li Jingyun, Li Lin
1 Department of AIDS Research, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology , Beijing, China .
AIDS Res Hum Retroviruses. 2015 May;31(5):559-63. doi: 10.1089/AID.2014.0337.
Multiple subtypes were found to be epidemic in the Shenzhen men who have sex with men (MSM) population, which always predicts the emergence of a unique recombinant. In 2012, CRF55_01B was first reported, which later was proven to have originated in MSM in Shenzhen city. In this study, we reported a unique recombinant form (URF) of HIV-1 identified in a man who has had sex with men in Shenzhen city. The strain showed a genomic schematic map similar to CRF55_01B with subtype C segments inserted in the gag and pol genes. The full-length genome was amplified in two halves with 1-kb overlap regions. The PCR products were cloned and sequenced. A recombination detection program showed that two subtype C fragments and two subtype B fragments were inserted into the CRF01_AE backbone genome in the gag and pol regions. In the phylogenetic tree, the subtype C fragments clustered with CRF07_BC variants and the other segments grouped with CRF55_01B strains except for one segment that clustered with CRF01_AE. Similar breakpoints between our strain and CRF65_cpx were also observed. The data suggested that the URF strain might be the recombinant form of CRF55_01B, CRF01_AE, and CRF07_BC. This is the first report of a third generation of recombination of HIV-1 that originated from CRF55_01B in China. The identification of the URF indicated the severity of the HIV epidemic in Shenzhen MSM and the urgent need for epidemiological surveillance of the new recombination.
研究发现多种亚型在深圳男男性行为者（MSM）人群中流行，这往往预示着一种独特重组体的出现。2012年，首次报告了CRF55_01B，后来证明其起源于深圳市的MSM。在本研究中，我们报告了在深圳市一名男男性行为者中鉴定出的一种独特的HIV-1重组形式（URF）。该毒株显示出与CRF55_01B相似的基因组示意图，gag和pol基因中插入了C亚型片段。全长基因组分两半扩增，重叠区域为1 kb。PCR产物进行克隆和测序。重组检测程序显示，两个C亚型片段和两个B亚型片段插入到gag和pol区域的CRF01_AE主干基因组中。在系统发育树中，C亚型片段与CRF07_BC变异株聚类，其他片段与CRF55_01B毒株聚类，但有一个片段与CRF01_AE聚类。在我们的毒株和CRF65_cpx之间也观察到了相似的断点。数据表明，URF毒株可能是CRF55_01B、CRF01_AE和CRF07_BC的重组形式。这是中国首次报道源自CRF55_01B的第三代HIV-1重组。URF的鉴定表明深圳MSM人群中HIV流行的严重性以及对新重组体进行流行病学监测的迫切需求。
