Betancourt Dillon, de Queiroz Nina M G P, Xia Tianli, Ahn Jeonghyun, Barber Glen N
Department of Cell Biology, University of Miami Miller School of Medicine, Miami, FL 33136.
Department of Cell Biology, University of Miami Miller School of Medicine, Miami, FL 33136
J Immunol. 2017 Apr 15;198(8):3023-3028. doi: 10.4049/jimmunol.1602180. Epub 2017 Mar 13.
Zika virus (ZIKV) has become a serious public health concern because of its link to brain damage in developing human fetuses. Recombinant vesicular stomatitis virus (rVSV) was shown to be a highly effective and safe vector for the delivery of foreign immunogens for vaccine purposes. In this study, we generated rVSVs (wild-type and attenuated VSV with mutated matrix protein [VSVm] versions) that express either the full length ZIKV envelope protein (ZENV) alone or include the ZENV precursor to the membrane protein upstream of the envelope protein, and our rVSV-ZIKV constructs showed efficient immunogenicity in murine models. We also demonstrated maternal protective immunity in challenged newborn mice born to female mice vaccinated with VSVm-ZENV containing the transmembrane domain. Our data indicate that rVSVm may be a suitable strategy for the design of effective vaccines against ZIKV.
寨卡病毒（ZIKV）因其与发育中的人类胎儿脑损伤有关，已成为一个严重的公共卫生问题。重组水疱性口炎病毒（rVSV）被证明是一种高效且安全的载体，可用于递送用于疫苗目的的外源免疫原。在本研究中，我们构建了rVSV（野生型和带有突变基质蛋白[VSVm]版本的减毒VSV），它们单独表达全长寨卡病毒包膜蛋白（ZENV），或在包膜蛋白上游包含膜蛋白的ZENV前体，并且我们的rVSV-ZIKV构建体在小鼠模型中显示出有效的免疫原性。我们还证明了，用含有跨膜结构域的VSVm-ZENV疫苗接种的雌性小鼠所生的新生小鼠在受到攻击时具有母体保护性免疫。我们的数据表明，rVSVm可能是设计针对ZIKV的有效疫苗的合适策略。
