油菜素内酯不敏感 2 型通过磷酸化纤维素合酶 1 负调控纤维素的合成。
BRASSINOSTEROID INSENSITIVE2 negatively regulates cellulose synthesis in by phosphorylating cellulose synthase 1.
机构信息
Department of Biology, Eidgenössiche Technische Hochschule Zurich, 8092 Zurich, Switzerland.
Department of Biochemistry and Molecular Biology, University of Nevada, Reno, NV 89557.
出版信息
Proc Natl Acad Sci U S A. 2017 Mar 28;114(13):3533-3538. doi: 10.1073/pnas.1615005114. Epub 2017 Mar 13.
Abstract
The deposition of cellulose is a defining aspect of plant growth and development, but regulation of this process is poorly understood. Here, we demonstrate that the protein kinase BRASSINOSTEROID INSENSITIVE2 (BIN2), a key negative regulator of brassinosteroid (BR) signaling, can phosphorylate cellulose synthase A1 (CESA1), a subunit of the primary cell wall cellulose synthase complex, and thereby negatively regulate cellulose biosynthesis. Accordingly, point mutations of the BIN2-mediated CESA1 phosphorylation site abolished BIN2-dependent regulation of cellulose synthase activity. Hence, we have uncovered a mechanism for how BR signaling can modulate cellulose synthesis in plants.
摘要
纤维素的沉积是植物生长和发育的一个决定性方面，但这一过程的调节机制还知之甚少。在这里，我们证明了蛋白激酶 BRASSINOSTEROID INSENSITIVE2（BIN2），一种油菜素内酯（BR）信号的关键负调控因子，可以磷酸化纤维素合酶 A1（CESA1），这是初生细胞壁纤维素合酶复合物的一个亚基，从而负调控纤维素的生物合成。因此，点突变 BIN2 介导的 CESA1 磷酸化位点消除了 BIN2 对纤维素合酶活性的依赖调节。因此，我们揭示了 BR 信号如何在植物中调节纤维素合成的机制。
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