Lai Francesco, Sinico Chiara, De Logu Alessandro, Zaru Marco, Müller Rainer H, Fadda Anna M
Dipartimento Farmaco Chimico Tecnologico, Università degli Studi di Cagliari, Cagliari, Italy.
Int J Nanomedicine. 2007;2(3):419-25.
The effect of SLN incorporation on transdermal delivery and in vitro antiherpetic activity of Artemisia arborescens essential oil was investigated. Two different SLN formulations were prepared using the hot-pressure homogenization technique, Compritol 888 ATO as lipid, and Poloxamer 188 and Miranol Ultra C32 as surfactants. Formulations were examined for their stability for two years by monitoring average size distribution and zeta potential values. The antiviral activity of free and SLN incorporated essential oil was tested in vitro against Herpes Simplex Virus-1 (HSV-1) by a quantitative tetrazolium-based colorimetric method (MTT), while the effects of essential oil incorporation into SLN on both the permeation through and the accumulation into the skin strata was investigated by using in vitro diffusion experiments through newborn pig skin and an almond oil Artemisia essential oil solution as a control. Results showed that both SLN formulations were able to entrap the essential oil in high yields and that the mean particle size increased only slightly after two years of storage, indicating a high physical stability. In vitro antiviral assays showed that SLN incorporation did not affect the essential oil antiherpetic activity. The in vitro skin permeation experiments demonstrated the capability of SLN of greatly improving the oil accumulation into the skin, while oil permeation occurred only when the oil was delivered from the control solution.
研究了纳米结构脂质载体（SLN）包封对青蒿精油经皮给药及体外抗疱疹活性的影响。采用热压匀化技术，以Compritol 888 ATO为脂质，泊洛沙姆188和米腊诺尔超C32为表面活性剂，制备了两种不同的SLN制剂。通过监测平均粒径分布和zeta电位值，对制剂进行了两年的稳定性考察。采用基于四氮唑的定量比色法（MTT）体外检测游离精油和包封于SLN中的精油对单纯疱疹病毒1型（HSV-1）的抗病毒活性，同时通过新生猪皮体外扩散实验，以杏仁油-青蒿精油溶液作为对照，研究了精油包封于SLN中对其透过皮肤及在皮肤各层中蓄积的影响。结果表明，两种SLN制剂均能高效包封精油，且储存两年后平均粒径仅略有增加，表明具有较高的物理稳定性。体外抗病毒试验表明，SLN包封不影响精油的抗疱疹活性。体外皮肤渗透实验表明，SLN能够显著提高精油在皮肤中的蓄积量，而只有当精油从对照溶液中释放时才会发生渗透。
