Residue Chemistry & Predictive Microbiology Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, United States.
Food Safety Intervention Technology Research Unit, U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, Wyndmoor, PA 19038, United States.
Food Res Int. 2017 Jan;91:55-62. doi: 10.1016/j.foodres.2016.11.029. Epub 2016 Nov 30.
The objective of this study was to investigate and evaluate the effects of high hydrostatic pressure (HHP) applied to cantaloupe puree (CP) on microbial loads and product quality during storage for 10days at 4°C. Freshly prepared, double sealed and double bagged CP (ca. 5g) was pressure treated at 300, 400 and 500MPa at 8°C and 15°C for 5min. Microflora populations, soluble solid content, pH, color, antioxidant activity, appearance and aroma were measured at 1, 6, and 10d of storage. Results showed that high pressure treatment of 300MPa (8°C and 15°C) resulted in reduction of total aerobic plate count from 3.3 to 1.8logCFU/g. The treatment reduced the populations of native aerobic plate count to non-detectable levels (detection limit 1logCFU/g) at 400MPa and 500MPa pressures at 15°C. Pressure treatment completely inactivated mold and yeast in puree below the limits of detection at day 1 and no regrowth was observed during 10days of storage at 4°C while mold and yeast in untreated puree survived during the storage. High pressure treatment did not show any adverse impact on physical properties as soluble solid content (SSC, 11.2°Brix) and acidity (pH, 6.9). The instrumental color parameters (L*, a*, b*) were affected due to HHP treatment creating a slightly lighter product, compared to control, as indicated by higher L.* and lower a* values. However the change was not detected by the sensory panel while evaluating appearance scores. Pressure treatment did not affect the antioxidant capacity of puree product compared to control. Visual appearance and sniffing aroma test by panel revealed no adverse changes in the sensory parameters as a result of HHP treatment. HHP method described in this study appears to be a promising way to inactivate spoilage microorganisms in the cantaloupe puree and maintain quality. This study provides a viable option for preservation and marketing this product.
本研究旨在探究并评估高压处理(HPP)对半切蜜瓜纯汁(CP)的微生物负荷和产品质量的影响,实验在 4°C 下贮藏 10 天。将新鲜制备的、双层密封和双层包装的 CP(约 5g)在 8°C 和 15°C 下以 300、400 和 500MPa 压力处理 5min。在贮藏的第 1、6 和 10 天,测量微生物种群、可溶性固形物含量、pH 值、颜色、抗氧化活性、外观和香气。结果表明,300MPa(8°C 和 15°C)高压处理使总需氧平板计数从 3.3 减少到 1.8logCFU/g。该处理使原生需氧平板计数在 400MPa 和 500MPa 压力下于 15°C 下降低至无法检测水平(检测限 1logCFU/g)。高压处理在第 1 天完全灭活纯汁中的霉菌和酵母,在 4°C 下贮藏 10 天期间未观察到再生长,而未经处理的纯汁中的霉菌和酵母在贮藏期间存活。高压处理对物理性质(可溶性固形物含量(SSC,11.2°Brix)和酸度(pH,6.9))没有任何不利影响。仪器颜色参数(L*、a*、b*)由于 HPP 处理导致产品稍微变浅而受到影响,与对照相比,L值更高,a值更低。然而,在评估外观评分时,感官小组没有检测到这种变化。与对照相比,高压处理对纯汁产品的抗氧化能力没有影响。通过小组的视觉外观和嗅闻香气测试,HPP 处理没有导致感官参数发生不利变化。本研究中描述的 HPP 方法似乎是一种在半切蜜瓜纯汁中灭活腐败微生物并保持质量的有前途的方法。该研究为该产品的保存和营销提供了可行的选择。