Klobutcher L A, Huff M E, Gonye G E
Department of Biochemistry, University of Connecticut Health Center, Farmington 06032.
Nucleic Acids Res. 1988 Jan 11;16(1):251-64. doi: 10.1093/nar/16.1.251.
During its life cycle, the hypotrichous ciliated protozoan Oxytricha nova transforms a copy of its micronucleus, which contains chromosome-sized DNA, into a macronucleus containing linear, gene-sized DNA molecules. A region of the micronuclear genome has been defined that gives rise to two distinct macronuclear DNA molecules during development. Through analysis of recombinant macronuclear and micronuclear clones, the generation of the two macronuclear DNA molecules was shown to be the result of alternative use of chromosome fragmentation sites. In addition, evidence was obtained that adjacent micronuclear precursors of macronuclear DNA molecules can overlap by a few base pairs. The significance of these findings in relation to developmental chromosome fragmentation is discussed.
在其生命周期中，具毛腹毛目纤毛虫新奥克特里虫将其包含染色体大小DNA的小核的一个副本转化为包含线性、基因大小DNA分子的大核。已定义了小核基因组的一个区域，该区域在发育过程中产生两个不同的大核DNA分子。通过对重组大核和小核克隆的分析，表明这两个大核DNA分子的产生是染色体断裂位点交替使用的结果。此外，还获得了证据，证明大核DNA分子相邻的小核前体可以重叠几个碱基对。讨论了这些发现与发育性染色体断裂相关的意义。
