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在新大核草履虫的大核发育过程中，反向末端重复序列被添加到基因中。

Inverted terminal repeats are added to genes during macronuclear development in Oxytricha nova.

作者信息

Boswell R E, Klobutcher L A, Prescott D M

出版信息

Proc Natl Acad Sci U S A. 1982 May;79(10):3255-9. doi: 10.1073/pnas.79.10.3255.

DOI:10.1073/pnas.79.10.3255
PMID:6808504
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC346394/
Abstract

In the hypotrichous ciliate Oxytricha nova all of the macronuclear DNA is in the form of low molecular weight, gene-sized molecules with an average size of 2,200 base pairs. These molecules are produced during macronuclear development by excision from micronuclear chromosomes. All, or nearly all, of the small macronuclear DNA molecules possess an inverted terminal repeat sequence consisting of 5' C4A4 3' repeats. The hypothesis that this terminal sequence serves as a recognition signal for excision of gene-sized molecules from chromosomes has been tested. A sequence containing the C4A4 repeat has been isolated and used to screen clones of micronuclear DNA for the presence of the repeat sequence. The results show that the intact repeated C4A4 sequence is not present at the ends of macronuclear sequences as they exist in the micronuclear chromosomes. Therefore, the entire terminal repeat is not a recognition sequence for gene excision but must be added to the ends of gene-sized molecules during or after the excision process.

摘要

在寡毛纲纤毛虫新奥克特里亚中，所有大核DNA均呈低分子量、基因大小的分子形式，平均大小为2200个碱基对。这些分子是在大核发育过程中通过从小核染色体上切除产生的。所有或几乎所有的小大核DNA分子都具有由5' C4A4 3'重复序列组成的反向末端重复序列。关于该末端序列作为从染色体上切除基因大小分子的识别信号的假说已经得到验证。一个包含C4A4重复序列的序列已被分离出来，并用于筛选小核DNA克隆中该重复序列的存在情况。结果表明，完整的重复C4A4序列并不存在于微核染色体中现存的大核序列末端。因此，整个末端重复序列不是基因切除的识别序列，而是必须在切除过程中或之后添加到基因大小分子的末端。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/587d/346394/9ac073f09e31/pnas00449-0202-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/587d/346394/4ddb3f9ded88/pnas00449-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/587d/346394/9d6fd92d30da/pnas00449-0201-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/587d/346394/156ba91a1a38/pnas00449-0201-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/587d/346394/d64bff5bad8c/pnas00449-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/587d/346394/9ac073f09e31/pnas00449-0202-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/587d/346394/4ddb3f9ded88/pnas00449-0201-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/587d/346394/9d6fd92d30da/pnas00449-0201-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/587d/346394/156ba91a1a38/pnas00449-0201-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/587d/346394/d64bff5bad8c/pnas00449-0202-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/587d/346394/9ac073f09e31/pnas00449-0202-b.jpg

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