含碱基类似物2-氨基腺嘌呤的DNA：制备、用作杂交探针及被限制性核酸内切酶切割
DNA containing the base analogue 2-aminoadenine: preparation, use as hybridization probes and cleavage by restriction endonucleases.
作者信息
Chollet A, Kawashima E
机构信息
Department of Chemistry, Biogen SA, Geneva, Switzerland.
出版信息
Nucleic Acids Res. 1988 Jan 11;16(1):305-17. doi: 10.1093/nar/16.1.305.
Abstract
The base analogue 2-aminoadenine (2,6-diaminopurine, D) has been introduced at selected positions into synthetic oligodeoxyribonucleotides and DNA by the combined use of chemical and enzymatic methods. 2-aminoadenine substitution for adenine introduces changes in the minor groove of DNA and creates an additional hydrogen bond in the Watson-Crick base pair with thymine. Oligonucleotide hybridization probes containing 2-aminoadenine showed increased selectivity and hybridization strength during DNA-DNA hybridization to phage or genomic target DNA. Properties of the base analogue with respect to DNA modifying enzymes were examined. 2-aminoadenine was used to probe minor groove determinants during the treatment of DNA by 12 restriction endonucleases. Inhibition of cleavage was found for several restriction enzymes.
摘要
通过化学和酶促方法的联合使用，碱基类似物2-氨基腺嘌呤（2,6-二氨基嘌呤，D）已被引入到合成的寡脱氧核糖核苷酸和DNA的特定位置。用2-氨基腺嘌呤取代腺嘌呤会引起DNA小沟的变化，并在与胸腺嘧啶形成的沃森-克里克碱基对中产生一个额外的氢键。含有2-氨基腺嘌呤的寡核苷酸杂交探针在与噬菌体或基因组靶DNA进行DNA-DNA杂交时显示出更高的选择性和杂交强度。研究了该碱基类似物相对于DNA修饰酶的性质。在12种限制性内切核酸酶处理DNA的过程中，用2-氨基腺嘌呤来探测小沟决定因素。发现几种限制性酶的切割受到抑制。
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