一种合成受保护的2-氨基嘌呤衍生物并将其掺入含有Eco RI和Bam HI识别位点的寡脱氧核苷酸中的新方法。
A new approach to the synthesis of a protected 2-aminopurine derivative and its incorporation into oligodeoxynucleotides containing the Eco RI and Bam HI recognition sites.
作者信息
McLaughlin L W, Leong T, Benseler F, Piel N
机构信息
Department of Chemistry, Boston College, Chestnut Hill, MA 02167.
出版信息
Nucleic Acids Res. 1988 Jun 24;16(12):5631-44. doi: 10.1093/nar/16.12.5631.
Abstract
A protected 2-aminopurine nucleoside suitable for incorporation into oligodeoxynucleotides using phosphite triester chemical synthesis procedures has been prepared via oxidation of a purine hydrazino derivative with silver (I) oxide. Five oligodeoxynucleotides containing Eco RI and Bam HI recognition sites have been prepared such that, in the double stranded form, the 2-aminopurine base has either a complementary thymine or cytosine nucleobase. The helix character and thermodynamic parameters for helix formation have been examined.
摘要
一种适合使用亚磷酸三酯化学合成方法掺入寡脱氧核苷酸中的受保护2-氨基嘌呤核苷，已通过用氧化银（I）氧化嘌呤肼衍生物制备而成。已制备了五种含有Eco RI和Bam HI识别位点的寡脱氧核苷酸，使得在双链形式中，2-氨基嘌呤碱基具有互补的胸腺嘧啶或胞嘧啶核碱基。已研究了螺旋特征和螺旋形成的热力学参数。
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