Morin G B, Cech T R
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309-0215.
Nucleic Acids Res. 1988 Jan 11;16(1):327-46. doi: 10.1093/nar/16.1.327.
Tetrahymena thermophila mitochondrial DNA is a linear molecule with two tRNAs, large subunit beta (LSU beta) rRNA (21S rRNA) and LSU alpha rRNA (5.8S-like RNA) encoded near each terminus. The DNA sequence of approximately 550 bp of this region was determined in six species of Tetrahymena. In three species the LSU beta rRNA and tRNA(leu) genes were not present on one end of the DNA, demonstrating a mitochondrial genome organization different from that of T. thermophila. The DNA sequence of the LSU alpha rRNA was used to construct a mitochondrial phylogenetic tree, which was found to be topologically equivalent to a phylogenetic tree based on nuclear small subunit rRNA sequences (Sogin et al. (1986) EMBO J. 5, 3625-3630). The mitochondrial rRNA gene was found to accumulate base-pair substitutions considerably faster than the nuclear rRNA gene, the rate difference being similar to that observed for mammals.
嗜热四膜虫的线粒体DNA是一种线性分子，在每个末端附近编码两个tRNA、大亚基β（LSUβ）rRNA（21S rRNA）和LSUα rRNA（5.8S样RNA）。测定了六种四膜虫该区域约550 bp的DNA序列。在三个物种中，LSUβ rRNA和tRNA（leu）基因不在DNA的一端，这表明其线粒体基因组组织与嗜热四膜虫不同。LSUα rRNA的DNA序列用于构建线粒体系统发育树，发现其拓扑结构与基于核小亚基rRNA序列构建的系统发育树相同（Sogin等人，（1986年）《欧洲分子生物学组织杂志》5，3625 - 3630）。发现线粒体rRNA基因积累碱基对替换的速度比核rRNA基因快得多，速率差异与在哺乳动物中观察到的相似。
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