一种缺乏“二氢尿嘧啶”环和茎的哺乳动物线粒体丝氨酸转运核糖核酸。
A mammalian mitochondrial serine transfer RNA lacking the "dihydrouridine" loop and stem.
作者信息
de Bruijn M H, Schreier P H, Eperon I C, Barrell B G, Chen E Y, Armstrong P W, Wong J F, Roe B A
出版信息
Nucleic Acids Res. 1980 Nov 25;8(22):5213-22. doi: 10.1093/nar/8.22.5213.
A unique transfer RNA has been identified in human and bovine mitochondria that lacks the "dihydrouridine" loop and stem structure. This tRNA is mitochondrially coded as shown by DNA sequence analysis of the human and bovine mitochondrial DNA. Sequence analysis of the RNA shows that it is post-transcriptionally modified by the addition of CCA at the 3' terminus and that at least one base is modified. As predicted by its anticodon (GCU, corresponding to the serine codons AGU/C) this tRNA can be aminoacylated with serine when purified mitochondria are incubated in a medium containing 3H-serine.
在人类和牛的线粒体中发现了一种独特的转运RNA，它缺乏“二氢尿嘧啶”环和茎结构。通过对人类和牛线粒体DNA的序列分析表明，这种tRNA是由线粒体编码的。对该RNA的序列分析表明，它在转录后通过在3'末端添加CCA进行修饰，并且至少有一个碱基被修饰。正如其反密码子（GCU，对应于丝氨酸密码子AGU/C）所预测的那样，当将纯化的线粒体在含有3H-丝氨酸的培养基中孵育时，这种tRNA可以被丝氨酸氨酰化。
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