Labriola J, Weiss I, Zapatero J, Suyama Y
Department of Biology, University of Pennsylvania, Philadelphia 19104.
Curr Genet. 1987;11(6-7):529-36. doi: 10.1007/BF00384616.
Extraction of RNA from Tetrahymena mitochondrial ribosomes yields several RNA species, including a "large" 21S molecule, a "small" 14S molecule, a 7S molecule, and other smaller RNAs. The molecular weight of the 14S rRNA indicates that it is about 1,300 bases in length. We have sequenced the 14S rRNA gene and, by aligning our sequence with that of the corresponding small rRNA from E. coli, find that the 14S rDNA is at least 1,635 bases in length. We propose, based on the results of hybridization studies, that this unexpected length is due to the presence of 7S RNA sequence within the 14S gene sequence. The 7S region is apparently lost from the 14S rRNA, yet is still a component of the ribosome.
从四膜虫线粒体核糖体中提取RNA可得到几种RNA分子，包括一个“大的”21S分子、一个“小的”14S分子、一个7S分子以及其他更小的RNA。14S rRNA的分子量表明其长度约为1300个碱基。我们已对14S rRNA基因进行了测序，并通过将我们的序列与大肠杆菌相应小rRNA的序列进行比对，发现14S rDNA的长度至少为1635个碱基。基于杂交研究的结果，我们提出，这种意外的长度是由于14S基因序列中存在7S RNA序列。7S区域显然从14S rRNA中丢失了，但仍然是核糖体的一个组成部分。
