Umeå Centre for Molecular Medicine, Umeå University, Umeå S-90187, Sweden.
Sci Data. 2017 Mar 14;4:170031. doi: 10.1038/sdata.2017.31.
A detailed understanding of pancreatic β-cell mass distribution is a key element to fully appreciate the pathophysiology of models of diabetes and metabolic stress. Commonly, such assessments have been performed by stereological approaches that rely on the extrapolation of two-dimensional data and provide very limited topological information. We present ex vivo optical tomographic data sets of the full β-cell mass distribution in cohorts of obese ob/ob mice and their lean controls, together with information about individual islet β-cell volumes, their three-dimensional coordinates and shape throughout the volume of the pancreas between 4 and 52 weeks of age. These data sets offer the currently most comprehensive public record of the β-cell mass distribution in the mouse. As such, they may serve as a quantitative and topological reference for the planning of a variety of in vivo or ex vivo experiments including computational modelling and statistical analyses. By shedding light on intra- and inter-lobular variations in β-cell mass distribution, they further provide a powerful tool for the planning of stereological sampling assessments.
详细了解胰腺 β 细胞质量分布是充分了解糖尿病和代谢应激模型病理生理学的关键要素。通常，此类评估是通过依赖于二维数据外推的体视学方法进行的，这些方法提供的拓扑信息非常有限。我们提供了肥胖 ob/ob 小鼠及其瘦对照小鼠的整个 β 细胞质量分布的离体光学层析数据组，以及关于个体胰岛 β 细胞体积、它们在胰腺中的三维坐标和形状的信息，这些信息在 4 至 52 周龄的整个胰腺体积中。这些数据集提供了目前关于小鼠 β 细胞质量分布的最全面的公共记录。因此，它们可以作为各种体内或体外实验（包括计算建模和统计分析）的定量和拓扑参考。通过揭示 β 细胞质量分布的叶内和叶间变化，它们进一步为体视学采样评估的规划提供了有力工具。
