Umeå Centre for Molecular Medicine, Umeå University, Umeå, Sweden.
The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Stockholm, Sweden.
Sci Data. 2022 Sep 10;9(1):558. doi: 10.1038/s41597-022-01546-5.
Mouse models for streptozotocin (STZ) induced diabetes probably represent the most widely used systems for preclinical diabetes research, owing to the compound's toxic effect on pancreatic β-cells. However, a comprehensive view of pancreatic β-cell mass distribution subject to STZ administration is lacking. Previous assessments have largely relied on the extrapolation of stereological sections, which provide limited 3D-spatial and quantitative information. This data descriptor presents multiple ex vivo tomographic optical image datasets of the full β-cell mass distribution in mice subject to single high and multiple low doses of STZ administration, and in glycaemia recovered mice. The data further include information about structural features, such as individual islet β-cell volumes, spatial coordinates, and shape as well as signal intensities for both insulin and GLUT2. Together, they provide the most comprehensive anatomical record of the effects of STZ administration on the islet of Langerhans in mice. As such, this data descriptor may serve as reference material to facilitate the planning, use and (re)interpretation of this widely used disease model.
链脲佐菌素 (STZ) 诱导的糖尿病小鼠模型可能是用于临床前糖尿病研究的最广泛使用的系统，这归因于该化合物对胰腺 β 细胞的毒性作用。然而，缺乏对 STZ 给药后胰腺 β 细胞质量分布的全面了解。以前的评估在很大程度上依赖于体视学切片的推断，而体视学切片提供的三维空间和定量信息有限。本数据描述提供了多个经 STZ 单次高剂量和多次低剂量给药以及血糖恢复后的小鼠完整 β 细胞质量分布的离体断层光学图像数据集。这些数据还包括有关结构特征的信息，例如单个胰岛 β 细胞体积、空间坐标和形状以及胰岛素和 GLUT2 的信号强度。这些信息共同提供了 STZ 给药对小鼠胰岛的最全面的解剖记录。因此，该数据描述符可用作参考材料，以方便规划、使用和（重新）解释这种广泛使用的疾病模型。
