Fujiwara F, Todo S, Imashuku S
Department of Pediatrics, Kyoto Prefectural University of Medicine, Japan.
Prostaglandins Leukot Med. 1987 Nov;30(1):37-49. doi: 10.1016/0262-1746(87)90023-0.
Two human neuroblastoma cell lines, NCG and GOTO, were used to study the cytotoxic effect of gamma linolenic acid (GLA). The cell growth inhibition of these culture cells by GLA was found to be associated with striking membrane fatty acid modification. When culture cells were exposed to 20 micrograms/ml and 60 micrograms/ml GLA for 48 hr, polyenoic acids in cell membrane phospholipids (PC, PE, PI, PS) and triglyceride significantly increased; 1.8-21.0 fold for NCG and 1.04-11.5 fold for GOTO, in association with decreased monoenoic acids. The most remarkable changes were; increase of C18:3, C20:3, C20:4 and decrease of C18:1. CoQ10 (50 micrograms/ml) and vitamin E (10 microM) shown to protect against the cytotoxic effect of GLA did not modify the incorporation of GLA into tumor cells. These results indicate that the antitumor effect of GLA is probably due to cellular dysfunction caused by fatty acid modification after GLA incorporation.
使用两种人类神经母细胞瘤细胞系，即NCG和GOTO，来研究γ-亚麻酸（GLA）的细胞毒性作用。发现GLA对这些培养细胞的生长抑制与显著的膜脂肪酸修饰有关。当培养细胞暴露于20微克/毫升和60微克/毫升的GLA中48小时时，细胞膜磷脂（PC、PE、PI、PS）和甘油三酯中的多不饱和脂肪酸显著增加；NCG增加了1.8至21.0倍，GOTO增加了1.04至11.5倍，同时单不饱和脂肪酸减少。最显著的变化是；C18:3、C20:3、C20:4增加，C18:1减少。已证明可防止GLA细胞毒性作用的辅酶Q10（50微克/毫升）和维生素E（10微摩尔）并未改变GLA掺入肿瘤细胞的情况。这些结果表明，GLA的抗肿瘤作用可能是由于GLA掺入后脂肪酸修饰导致的细胞功能障碍。
