Mengeaud V, Nano J L, Fournel S, Rampal P
Laboratoire de Gastroentéologie et de Nutrition, UER de Médecine, Nice, France.
Prostaglandins Leukot Essent Fatty Acids. 1992 Dec;47(4):313-9. doi: 10.1016/0952-3278(92)90204-v.
Several studies have demonstrated that certain essential fatty acids present a specific cytotoxicity for tumor cells. However, no investigation of this type has been performed on human colon cancer cells to date. This study investigated the effect of gamma-linolenic acid (GLA), eicosapentaenoic acid (EPA) and prostaglandin (PG) E1 on the proliferation and metabolism of three human colon cancer cell lines: HT 29, HRT 18, and CACO 2. GLA, EPA and PGE1 all inhibited the proliferation of the three cell lines, but with a decreasing gradient of sensitivity: HRT 18 > HT 29 > CACO 2, and with different IC50 values. PGE1 was markedly less effective than the other two. GLA and EPA increased lipid peroxidation and membrane fluidity in a dose-dependent manner. The presence of indomethacin did not modify the effects of GLA and EPA. In addition, PGE1 had little effect on membrane fluidity and lipid peroxidation. The antitumoral effect thus does not appear to be mediated by PGE1. Addition of vitamin E decreased the effects of GLA and EPA, which supports the hypothesis of direct action by these fatty acids. In conclusion, while EPA and GLA have an antitumoral effect in vitro, their effect on primary cultures of normal human colon cells must be investigated to determine whether this effect is specific to tumoral cells, as has been observed for other cell types.
多项研究表明，某些必需脂肪酸对肿瘤细胞具有特定的细胞毒性。然而，迄今为止尚未对人类结肠癌细胞进行此类研究。本研究调查了γ-亚麻酸（GLA）、二十碳五烯酸（EPA）和前列腺素（PG）E1对三种人类结肠癌细胞系HT 29、HRT 18和CACO 2增殖和代谢的影响。GLA、EPA和PGE1均抑制这三种细胞系的增殖，但敏感性呈递减梯度：HRT 18 > HT 29 > CACO 2，且IC50值不同。PGE1的效果明显低于其他两者。GLA和EPA以剂量依赖的方式增加脂质过氧化和膜流动性。吲哚美辛的存在并未改变GLA和EPA的作用。此外，PGE1对膜流动性和脂质过氧化影响很小。因此，抗肿瘤作用似乎不是由PGE1介导的。添加维生素E可降低GLA和EPA的作用，这支持了这些脂肪酸直接作用的假说。总之，虽然EPA和GLA在体外具有抗肿瘤作用，但必须研究它们对正常人结肠细胞原代培养的影响，以确定这种作用是否像在其他细胞类型中观察到的那样对肿瘤细胞具有特异性。
