Uchida I, Hashimoto K, Makino S, Sasakawa C, Yoshikawa M, Terakado N
National Institute of Animal Health, Tsukuba Science City, Ibaraki, Japan.
Plasmid. 1987 Sep;18(2):178-81. doi: 10.1016/0147-619x(87)90047-3.
The capsule plasmid pTE702 of Bacillus anthracis has been physically mapped with the restriction endonucleases HindIII, PstI, BamHI, SalI, and XhoI. A HindIII fragment map of pTE702 (96.5 kb) was obtained by analysis of the recombinant plasmids and cosmids containing overlapping fragments partially digested with HindIII. The physical map for PstI, BamHI, SalI, and XhoI was obtained by double digestion mapping of these sites in relation to the HindIII sites. The replication region of pTE702 was determined by in vitro genetic replicon labeling in B. subtilis.
炭疽芽孢杆菌的荚膜质粒pTE702已用限制性内切酶HindIII、PstI、BamHI、SalI和XhoI进行了物理图谱绘制。通过分析含有用HindIII部分消化的重叠片段的重组质粒和黏粒，获得了pTE702（96.5 kb）的HindIII片段图谱。PstI、BamHI、SalI和XhoI的物理图谱是通过将这些位点与HindIII位点进行双酶切图谱分析获得的。pTE702的复制区域通过枯草芽孢杆菌中的体外遗传复制子标记来确定。
