牛疱疹病毒1型DNA的PstI片段的分子克隆与特性分析
Molecular cloning and characterization of PstI fragments of bovine herpesvirus-1 DNA.
作者信息
Gupta P K, Rai A
机构信息
National Biotechnology Center, Indian Veterinary Research Institute, Izatnagar.
出版信息
Biochem Mol Biol Int. 1993 Jul;30(3):443-52.
The DNA from an Indian isolate of BHV-1 was cleaved with the restriction enzyme PstI and cloned into pUC9 plasmid. Two recombinant plasmids were then analyzed using restriction endonucleases PstI, EcoRI, BamHI, SmaI, SalI, HindIII, BglI, HaeIII, XhoI, RsaI and StuI. On the basis of digested fragments of recombinant plasmids, two plasmids could be mapped for restriction enzymes SalI, StuI, SmaI and XhoI using single and double digestion.
用限制性内切酶PstI切割来自印度BHV - 1分离株的DNA，并将其克隆到pUC9质粒中。然后使用限制性内切酶PstI、EcoRI、BamHI、SmaI、SalI、HindIII、BglI、HaeIII、XhoI、RsaI和StuI对两个重组质粒进行分析。根据重组质粒的酶切片段，可通过单酶切和双酶切对两个质粒进行SalI、StuI、SmaI和XhoI限制性酶切图谱绘制。
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