Koplow J, Byrne M C, Jen G, Tempé J, Chilton M D
Plasmid. 1984 Jan;11(1):17-27. doi: 10.1016/0147-619x(84)90003-9.
Virulence of Agrobacterium rhizogenes, agent of hairy root disease, is conferred by large plasmids called Ri (root-inducing) plasmids. We have determined the BamHI fragment map of pRi8196, MW 143 Mda, principally by analysis of recombinant plasmids containing overlapping BamHI partial-digest fragments. Clones containing solitary BamHI inserts of remaining unmapped fragments were used to probe a series of Southern-blotted, pRi8196-derived EcoRI, PstI, HindIII, SalI, or SmaI digests. Continguous hybridized bands represented complements of EcoRI, PstI, HindIII, SalI, or SmaI fragments which bridged the unmapped BamHI fragments. We present, in addition, a detailed map of the core T-DNA region with respect to the restriction endonucleases SalI, EcoRI, HpaI, and HindIII.
发根农杆菌是毛根病的病原体，其毒性由一种称为Ri（发根诱导）质粒的大质粒赋予。我们主要通过分析含有重叠BamHI部分消化片段的重组质粒，确定了分子量为143 Mda的pRi8196的BamHI片段图谱。含有剩余未定位片段的单个BamHI插入片段的克隆被用于探测一系列经Southern印迹法处理的、源自pRi8196的EcoRI、PstI、HindIII、SalI或SmaI消化产物。相邻的杂交带代表了连接未定位BamHI片段的EcoRI、PstI、HindIII、SalI或SmaI片段的互补片段。此外，我们还给出了核心T-DNA区域相对于限制性内切酶SalI、EcoRI、HpaI和HindIII的详细图谱。
