Manakul Patcharaporn, Peerakietkhajorn Saranya, Matsuura Tomoaki, Kato Yasuhiko, Watanabe Hajime
Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan.
Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka, Japan.
Mar Environ Res. 2017 Jul;128:70-75. doi: 10.1016/j.marenvres.2017.03.001. Epub 2017 Mar 6.
The crustacean zooplankton Daphnia magna has been widely used for chemical toxicity tests. Although abiotic factors have been well documented in ecotoxicological test protocols, biotic factors that may affect the sensitivity to chemical compounds remain limited. Recently, we identified symbiotic bacteria that are critical for the growth and reproduction of D. magna. The presence of symbiotic bacteria on Daphnia raised the question as to whether these bacteria have a positive or negative effect on toxicity tests. In order to evaluate the effects of symbiotic bacteria on toxicity tests, bacteria-free Daphnia were prepared, and their chemical sensitivities were compared with that of Daphnia with symbiotic bacteria based on an acute immobilization test. The Daphnia with symbiotic bacteria showed higher chemical resistance to nonylphenol, fenoxycarb, and pentachlorophenol than bacteria-free Daphnia. These results suggested potential roles of symbiotic bacteria in the chemical resistance of its host Daphnia.
甲壳类浮游动物大型溞已被广泛用于化学毒性测试。尽管非生物因素在生态毒理学测试方案中已有详细记录，但可能影响对化合物敏感性的生物因素仍然有限。最近，我们鉴定出了对大型溞的生长和繁殖至关重要的共生细菌。大型溞上共生细菌的存在引发了一个问题，即这些细菌对毒性测试有正面还是负面影响。为了评估共生细菌对毒性测试的影响，制备了无菌大型溞，并基于急性固定试验将其化学敏感性与有共生细菌的大型溞进行了比较。有共生细菌的大型溞对壬基酚、苯氧威和五氯酚的化学抗性高于无菌大型溞。这些结果表明共生细菌在其宿主大型溞的化学抗性中可能发挥的作用。
