褪黑素衍生物对人牙龈成纤维细胞的抗氧化作用。

The anti-oxidant effects of melatonin derivatives on human gingival fibroblasts.

作者信息

Phiphatwatcharaded Chawapon, Puthongking Ploenthip, Chaiyarit Ponlatham, Johns Nutjaree Pratheepawanit, Sakolchai Sumon, Mahakunakorn Pramote

机构信息

Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand; Melatonin Research Group, Khon Kaen University, Khon Kaen 40002, Thailand.

Research Group of Chronic Inflammatory Oral Diseases and Systemic Diseases Associated with Oral Health, Khon Kaen University, Khon Kaen, 40002, Thailand; Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand.

出版信息

Arch Oral Biol. 2017 Jul;79:55-61. doi: 10.1016/j.archoralbio.2017.02.022. Epub 2017 Mar 7.

DOI:10.1016/j.archoralbio.2017.02.022

PMID:28292674

Abstract

OBJECTIVES

Aim of this in vitro study was to evaluate the anti-oxidant activity of indole ring modified melatonin derivatives as compared with melatonin in primary human gingival fibroblast (HGF) cells.

METHODS

Anti-oxidant activity of melatonin (MLT), acetyl-melatonin (AMLT) and benzoyl-melatonin (BMLT) was evaluated by5 standard methods as follows: 2, 2-diphenyl-1-picrylhydrazyl (DPPH); ferric ion reducing antioxidant power (FRAP); superoxide anion scavenging; nitric oxide (NO) scavenging; and thiobarbituric acid reactive substances (TBARs).Evaluation of cellular antioxidant activity (CAA) and protectivity against HO induced cellular damage was performed via MTT assay in HGF cells.

RESULTS

According to the standard anti-oxidant assays, the antioxidant power of AMLT and BMLT were slightly less than MLT in FRAP and superoxide scavenging assays. In the NO scavenging and TBARs assays, BMLT and AMLT were more potent than MLT, whereas DPPH assays demonstrated that MLT was more potent than others. BMLT and AMLT had more potent anti-oxidant and protective activities against HOin HGF cells as compared with MLT.

CONCLUSIONS

MLT derivatives demonstrated different anti-oxidant activities as compared with MLT, depending upon assays. These findings imply that N-indole substitution of MLT may help to improve hydrogen atom transfer to free radicals but electron transfer property is slightly decreased. Anti-oxidant and protective effects of melatonin derivatives (AMLT and BMLT) on human gingival fibroblasts imply the potential use of these molecules as alternative therapeutics for chronic inflammatory oral diseases.

摘要

目的

本体外研究的目的是评估吲哚环修饰的褪黑素衍生物与褪黑素相比在原代人牙龈成纤维细胞（HGF）中的抗氧化活性。

方法

通过以下5种标准方法评估褪黑素（MLT）、乙酰褪黑素（AMLT）和苯甲酰褪黑素（BMLT）的抗氧化活性：2,2-二苯基-1-苦基肼（DPPH）；铁离子还原抗氧化能力（FRAP）；超氧阴离子清除；一氧化氮（NO）清除；以及硫代巴比妥酸反应性物质（TBARs）。通过MTT法在HGF细胞中评估细胞抗氧化活性（CAA）和对HO诱导的细胞损伤的保护作用。