Zhang Yi, Guo Wen, Yue Zhenfeng, Lin Li, Zhao Fengjuan, Chen Peijin, Wu Weidong, Zhu Hong, Yang Bo, Kuang Yanyun, Wang Jiong
Food Inspection and Quarantine Technology Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau of China, Shenzhen, Guangdong, PR China.
Food Inspection and Quarantine Technology Center, Shenzhen Entry-Exit Inspection and Quarantine Bureau of China, Shenzhen, Guangdong, PR China; School of Chemistry and Environment Engineering Shenzhen University, Shenzhen, Guangdong, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Apr 15;1051:41-53. doi: 10.1016/j.jchromb.2017.01.026. Epub 2017 Jan 21.
In this paper, a simple, rapid, solvent-less and environmental friendliness microextraction method, microwave-assisted extraction-hollow fiber-liquid/solid phase microextraction (MAE-HF-L/SME), was developed for simultaneous extraction and enrichment of 54 trace hydrophilic/lipophilic pharmaceutical and personal care products (PPCPs) from fish samples. A solid-phase extraction material, solid-phase microextraction (SPME) fiber, was synthesized. The SPME fiber had a homogeneous, loose structure and good mechanical properties, and they exhibited a good adsorption capacity for most PPCPs selected. The material formed the basis for the method of MAE-HF-L/SME. A method of liquid chromatography-high resolution mass spectroscopy (LC-HRMS) for analysis of 54 PPCPs. Under optimal synthesis and extraction conditions, the limits of detection (LODs, n=3) and the limits of quantitation (LOQs, n=10) for the 54 PPCPs were between 0.01-0.50μg·kg and 0.052.00μg·kg, respectively. Percent recoveries and the relative standard deviations (RSDs) in spiked fish samples (n=6) were between 56.3%-119.9% and 0.3%-17.1%, respectively. The microextraction process of 54 PPCPs in MAE-HF-L/SME took approximately 12min. The method has a low matrix interference and high enrichment factor and may be applicable for determination of 54 different PPCPs in fish samples.
本文开发了一种简单、快速、无溶剂且环境友好的微萃取方法——微波辅助萃取-中空纤维液/固相微萃取（MAE-HF-L/SME），用于同时从鱼样中萃取和富集54种痕量亲水性/亲脂性药物及个人护理用品（PPCPs）。合成了一种固相萃取材料——固相微萃取（SPME）纤维。该SPME纤维结构均匀、疏松，具有良好的机械性能，对所选的大多数PPCPs表现出良好的吸附能力。该材料构成了MAE-HF-L/SME方法的基础。建立了一种用于分析54种PPCPs的液相色谱-高分辨质谱（LC-HRMS）方法。在最佳合成和萃取条件下，54种PPCPs的检测限（LODs，n=3）和定量限（LOQs，n=10）分别在0.01 - 0.50μg·kg和0.05 - 2.00μg·kg之间。加标鱼样（n=6）中的回收率和相对标准偏差（RSDs）分别在56.3% - 119.9%和0.3% - 17.1%之间。MAE-HF-L/SME中54种PPCPs的微萃取过程约需12分钟。该方法具有低基质干扰和高富集倍数，可适用于鱼样中54种不同PPCPs的测定。
